Analisis Lactat Dehydrogenase dalam Serum Darah Menggunakan Sentrifugasi
Abstract
This study shortens the laboratory service time by shortening the centrifuge time and obtaining serum as an examination material. The research method used was pre-experimental research. The results showed that the average LDH in the blood group centrifuged 3000 rpm 5 minutes was 309.9 U / L and 306.4 U / L centrifuged 4400 rpm 3 minutes in the blood group. In conclusion, there is no difference in serum LDH levels from blood centrifuged at 3000 rpm for 5 minutes and 4400 rpm for 3 minutes.
Keywords: Centrifugation, Lactate Dehydrogenase, TAT
References
Brassard, D., Clime, L., Daoud, J., Geissler, M., Malic, L., Charlebois, D., & Veres, T. (2018). Microfluidic-Based Platform for Universal Sample Preparation and Biological Assays Automation for Life-Sciences Research and Remote Medical Applications. Deep Space Gateway Science Workshop 2018
Cadamuro, J., Mrazek, C., Leichtle, A. B., Kipman, U., Felder, T. K., Wiedemann, H., & Haschke-Becher, E. (2018). Influence of Centrifugation Conditions on the Results of 77 Routine Clinical Chemistry Analytes using Standard Vacuum Blood Collection Tubes and the New BD-Barricor Tubes. Biochemia Medica, 28(1), 010704. https://doi.org/10.11613/BM.2018.010704
Eid-Lidt, G., Reyes, J., Rivera, A., Limon, C., Soto, M., & Gaspar, J. (2019). TCT-777 Prevention of Radial Artery Occlusion: Comparison of 3 Hemostatic Methods in Transradial Intervention. Journal of the American College of Cardiology, 74(13), B761. DOI: 10.1016/j.jacc.2019.08.920
Ghani, L., Susilawati, M. D., & Novriani, H. (2016). Faktor Risiko Dominan Penyakit Jantung Koroner di Indonesia. Buletin Penelitian Kesehatan, 44(3). https://doi.org/10.22435/bpk.v44i3.5436.153-164
Gressner, A. M., & Gressner, O. A. (2017). International Federation of Clinical Chemistry and Laboratory Medicine. Lexikon der Medizinischen Laboratoriumsdiagnostik. https://doi.org/10.1007/978-3-662-49054-9_1601-1
Hermayanti, D., & Nursiloningrum, E. (2018). Hiperglikemia pada Anak dengan Diagnosis Diabetes Mellitus Type-1, Diferential Diagnostic Maturity Onset Diabetes of The Young (Mody). Saintika Medika, 14(2). https://doi.org/10.22219/sm.vol14.smumm2.7162
Kemenkes RI. (2019). Info Datin Situasi Kesehatan Jantung. Journal of Chemical Information and Modeling. https://doi.org/10.1017/CBO9781107415324.004
Lippi, G., Mattiuzzi, C., & Bovo, C. (2018). Are we Getting Better at the Preanalytical Phase or Just Better at Measuring it? Journal of Laboratory and Precision Medicine, 3, 11. https://doi.org/10.21037/jlpm.2018.01.03
Lokhandwala, P. M., Riel, S. L., Haley, L., Lu, C., Chen, Y., Silberstein, J., & Eshleman, J. R. (2017). Analytical Validation of Androgen Receptor Splice Variant 7 Detection in a Clinical Laboratory Improvement Amendments (CLIA) Laboratory Setting. Journal of Molecular Diagnostics, 18(1), 115-125. https://doi.org/10.1016/j.jmoldx.2016.08.003
Magnette, A., Chatelain, M., Chatelain, B., Ten Cate, H., & Mullier, F. (2016). Pre-Analytical Issues in the Haemostasis Laboratory: Guidance for the Clinical Laboratories. Thrombosis Journal, 14(1). https://doi.org/10.1186/s12959-016-0123-z
Ramadhani, Q. A., Garini, A., Nurhayati, N., & Harianja, S. H. (2019). Perbedaan Kadar Glukosa Darah Sewaktu Menggunakan Serum dan Plasma Edta. JPP (Jurnal Kesehatan Poltekkes Palembang), 14(2), 80-84. https://doi.org/10.36086/jpp.v14i2.407
Schneider, F., Maurer, C., & Friedberg, R. C. (2017). International Organization for Standardization (ISO) 15189. Annals of Laboratory Medicine, 37(5), 365-370. https://doi.org/10.3343/alm.2017.37.5.365
Schneider, S. A., & Alcalay, R. N. (2017). Neuropathology of Genetic Synucleinopathies with Parkinsonism: Review of the literature. Movement Disorders, 32(11), 1504-1523. https://doi.org/10.1002/mds.27193
Cadamuro, J., Mrazek, C., Leichtle, A. B., Kipman, U., Felder, T. K., Wiedemann, H., & Haschke-Becher, E. (2018). Influence of Centrifugation Conditions on the Results of 77 Routine Clinical Chemistry Analytes using Standard Vacuum Blood Collection Tubes and the New BD-Barricor Tubes. Biochemia Medica, 28(1), 010704. https://doi.org/10.11613/BM.2018.010704
Eid-Lidt, G., Reyes, J., Rivera, A., Limon, C., Soto, M., & Gaspar, J. (2019). TCT-777 Prevention of Radial Artery Occlusion: Comparison of 3 Hemostatic Methods in Transradial Intervention. Journal of the American College of Cardiology, 74(13), B761. DOI: 10.1016/j.jacc.2019.08.920
Ghani, L., Susilawati, M. D., & Novriani, H. (2016). Faktor Risiko Dominan Penyakit Jantung Koroner di Indonesia. Buletin Penelitian Kesehatan, 44(3). https://doi.org/10.22435/bpk.v44i3.5436.153-164
Gressner, A. M., & Gressner, O. A. (2017). International Federation of Clinical Chemistry and Laboratory Medicine. Lexikon der Medizinischen Laboratoriumsdiagnostik. https://doi.org/10.1007/978-3-662-49054-9_1601-1
Hermayanti, D., & Nursiloningrum, E. (2018). Hiperglikemia pada Anak dengan Diagnosis Diabetes Mellitus Type-1, Diferential Diagnostic Maturity Onset Diabetes of The Young (Mody). Saintika Medika, 14(2). https://doi.org/10.22219/sm.vol14.smumm2.7162
Kemenkes RI. (2019). Info Datin Situasi Kesehatan Jantung. Journal of Chemical Information and Modeling. https://doi.org/10.1017/CBO9781107415324.004
Lippi, G., Mattiuzzi, C., & Bovo, C. (2018). Are we Getting Better at the Preanalytical Phase or Just Better at Measuring it? Journal of Laboratory and Precision Medicine, 3, 11. https://doi.org/10.21037/jlpm.2018.01.03
Lokhandwala, P. M., Riel, S. L., Haley, L., Lu, C., Chen, Y., Silberstein, J., & Eshleman, J. R. (2017). Analytical Validation of Androgen Receptor Splice Variant 7 Detection in a Clinical Laboratory Improvement Amendments (CLIA) Laboratory Setting. Journal of Molecular Diagnostics, 18(1), 115-125. https://doi.org/10.1016/j.jmoldx.2016.08.003
Magnette, A., Chatelain, M., Chatelain, B., Ten Cate, H., & Mullier, F. (2016). Pre-Analytical Issues in the Haemostasis Laboratory: Guidance for the Clinical Laboratories. Thrombosis Journal, 14(1). https://doi.org/10.1186/s12959-016-0123-z
Ramadhani, Q. A., Garini, A., Nurhayati, N., & Harianja, S. H. (2019). Perbedaan Kadar Glukosa Darah Sewaktu Menggunakan Serum dan Plasma Edta. JPP (Jurnal Kesehatan Poltekkes Palembang), 14(2), 80-84. https://doi.org/10.36086/jpp.v14i2.407
Schneider, F., Maurer, C., & Friedberg, R. C. (2017). International Organization for Standardization (ISO) 15189. Annals of Laboratory Medicine, 37(5), 365-370. https://doi.org/10.3343/alm.2017.37.5.365
Schneider, S. A., & Alcalay, R. N. (2017). Neuropathology of Genetic Synucleinopathies with Parkinsonism: Review of the literature. Movement Disorders, 32(11), 1504-1523. https://doi.org/10.1002/mds.27193
Published
2020-12-30
Section
Articles
Copyright (c) 2020 Rosnita Sebayang, Yuana Idawati, Hotman Sinaga
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
