Analisis Vitamin C Menggunakan Metode Spektrofotometri UV-Vis (Tinjauan Literatur dan Aplikasi)
Abstract
Tujuan dari tinjauan literatur ini adalah untuk mengevaluasi penerapan metode spektrofotometri UV-Vis dalam analisis kandungan vitamin C pada berbagai spesies. Metode yang digunakan adalah identifikasi dan seleksi makalah yang relevan dari database akademik dan jurnal ilmiah. Makalah ditinjau berdasarkan judul jurnal, validitas metodologi, dan relevansi materi pelajaran. Hasil penelitian menunjukkan bahwa metode spektrofotometri UV-Vis telah berhasil digunakan untuk mengukur kadar vitamin C dalam berbagai bentuk seperti buah-buahan, jus, dan sediaan obat. Beberapa penelitian mengembangkan metode penelitian baru dengan menggabungkan teknologi atau bidang lain untuk meningkatkan presisi dan pemahaman. Parameter yang paling sering diperiksa meliputi panjang gelombang maksimum, batas deteksi, batas pengukuran, batas garis, dan presisi. Simpulan ini bahwa metode spektrofotometri UV-Vis efektif untuk analisis kuantitatif vitamin C pada spesies berbeda, dengan sensitivitas dan sensitivitas yang wajar. Metode baru terus dikembangkan untuk meningkatkan kinerja analitis sistem ini.
Kata Kunci: Vitamin C, Spektrofotometri UV-Vis
References
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Astiti, N. P. A., & Yulihastuti, D. A. (2018). Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata). Journal of Advances in Tropical Biodiversity and EnvironmentalSciences. 1(2), 33-35. https://simdos.unud.ac.id/uploads/file_penelitian_1_dir/33aac52642bc92b6501b7d00ad12d7d6.pdf
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Baits, M., Jahri, R. M., Suhaenah, A., Aminah, A., Naid, T., & Fawwaz, M. (2022). The Effect of Heating on Ascorbic Acid Levels of Brassica Oleracea L. Determined by UV-Visible Spectrophotometry. Pharmaceutical Reports, 1(1). https://doi.org/10.33096/pharm%20rep.v1i1.156
Bartzatt, R., & Wol, T. (2014). Detection and Assay of Vitamin B-2 (Riboflavin) in Alkaline Borate Buffer with UV/Visible Spectrophotometry. International scholarly research notices, 2014, 453085. https://doi.org/10.1155/2014/453085
Bi, H., Fernandes, A. C., Cardoso, S., & Freitas, P. (2016). Interference-Blind Microfluidic Sensor for Ascorbic acid Determination by UV/vis Spectroscopy. Sensors and Actuators B: Chemical, 224, 668-675. https://core.ac.uk/download/pdf/43253003.pdf
Carr, A. C., & Maggini, S. (2017). Vitamin C and Immune Function. Nutrients, 9(11), 1211. https://doi.org/10.3390/nu9111211
Chandra, B., & Putri, W. D. (2019). Penetapan Kadar Vitamin C Dan B1 Pada Buah Naga Merah (Hylocereus Lemairel (Hook.) Britton & Rose) dengan Metode Spektrofotometri Uv-Vis. Jurnal Farmasi Higea, 11(1), 62-74. http://dx.doi.org/10.52689/higea.v11i1.215
Devolli, A., Stafasani, M., Shahinasi, E., Dara, F., & Hamiti, H. (2021). Determination of Vitamin C Content in Commercial Fruit Juices by Volumetric and Spectrophotometric Methods. Journal of Hygienic Engineering & Design, 34. https://www.semanticscholar.org/paper/Determination-Of-Vitamin-C-Content-In-Commercial-By-Devolli-Stafasani/88cbe990659f99a336bb764d05158d1c3ad98bf0
Dewi, A. P. (2018). Penetapan Kadar Vitamin C dengan Spektrofotometri UV-Vis Pada Berbagai Variasi Buah Tomat. JOPS (Journal Of Pharmacy and Science), 2(1), 9-13. http://dx.doi.org/10.36341/jops.v2i1.1015
Febrianti, A., Aina, G. Q., & Farpina, E. (2022). Determination of Vitamin C and ß-Carotene Levels in Several Types of Chili (Capsicum sp) Using UV-Vis Spectrophotometry Method. Formosa Journal of Science and Technology, 1(8), 1129-1142. https://doi.org/10.55927/fjst.v1i8.1949
Fitriana, Y. A. N., & Fitri, A. S. (2020). Analisis Kadar Vitamin C pada Buah Jeruk Menggunakan Metode Titrasi Iodometri. Sainteks, 17(1), 27-32. http://dx.doi.org/10.30595/sainteks.v17i1.8530
Iskandar, S. R., Yuniarto, P. F., & Sulistyowati, Y. (2023). Analysis Of Vitamin C Levels Of Curry Leaf Ethanol Extract (Murraya Koenigii L. Spreng) By Uv-Vis Spectrophotometry. Al Makki Health Informatics Journal, 1(2), 50-59. https://doi.org/10.57185/hij.v1i2.7
Jutkus, R. A., Li, N., Taylor, L. S., & Mauer, L. J. (2015). Effect of Temperature and Initial Moisture Content on the Chemical Stability and Color Change of Various Forms of Vitamin C. International Journal of Food Properties, 18(4), 862-879. https://doi.org/10.1080/10942912.2013.805770
Khasanah, N. U., & Riyanto, R. (2023). Analysis of Ascorbic Acid Content in Pineapples and Melons Using the Uv-Vis Spectrophotometry Method. Indonesian Journal of Applied Science and Engineering, 1(1), 11-17. https://e-journal.ptti.info/index.php/ijase/article/view/52/60
Lavilla, I., Romero, V., Costas, P., & Bendicho, C. (2023). Kinetic Spectrophotometric Assay for the Determination of Vitamin C in Cosmetics Following Ultrasound-Assisted Emulsification. Analytical Methods, 15(7), 951-958. https://doi.org/10.1039/D2AY01795D
Pakaya, D. (2014). Peranan Vitamin C pada Kulit. Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan, 1(2), 45-54. https://123dok.com/document/zlnj6j6q-peranan-vitamin-pakaya-tadulako-kedokteran-fakultas-kedokteran-kesehatan.html
Passos, M. L., & Saraiva, M. L. M. (2019). Detection in UV-Visible Spectrophotometry: Detectors, Detection Systems, and Detection Strategies. Measurement, 135, 896-904. https://ui.adsabs.harvard.edu/link_gateway/2019Meas..135..896P/doi:10.1016/j.measurement.2018.12.045
Putri, M. P. (2023). Variation of Processed Fresh Pineapple on Vitamin C Content Using UV-VIS Spectrophotometry. Journal of Natural Sciences and Learning, 2(1), 29-33. https://journalng.uwks.ac.id/jnsl/article/view/59
Ramírez-Cahero, H. F., & Valdivia-López, M. A. (2018). Effect of Gamma Radiation on Sugars and Vitamin C: Radiolytic Pathways. Food chemistry, 245, 1131–1140. https://doi.org/10.1016/j.foodchem.2017.11.057
Rashid, M. U., Bhuiyan, M. K. H., & Quayum, M. E. (2013). Synthesis of SILVER Nano Particles (Ag-NPs) and Their Uses for Quantitative Analysis of Vitamin C Tablets. Dhaka University Journal of Pharmaceutical Sciences, 12(1), 29-33. http://dx.doi.org/10.3329/dujps.v12i1.16297
Riscahyani, N. M. ., Ekawati, E. R., & Ngibad, K. (2019). Identification of Ascorbic Acid Content in Carica Papaya l. Using Iodimetry and Uv-Vis Spectrophotometry. Indonesian Journal of Medical Laboratory Science and Technology, 1(2), 58–64. https://doi.org/10.33086/ijmlst.v1i2.1291
Rosmainar, L., Ningsih, W., Ayu, N. P., & Nanda, H. (2018). Penentuan Kadar Vitamin C Beberapa Jenis Cabai (Capsicum SP.) dengan Spektrofotometri UV-VIS. Jurnal Kimia Riset, 3(1), 1-5. https://doi.org/10.20473/jkr.v3i1.8874
Saeed, A. M., Al-kadumi, A. S., & Ali, N. J. M. (2018). Colorimetric Determination of Antioxidant Vitamins E and C. Pakistan Journal of Biotechnology, 15(2), 451-457. https://pjbt.org/index.php/pjbt/article/view/416
Saeed, A. M., Hamzah, M. J., & Ali, N. J. M. (2018). Sensitive Spectrophotometric Method for Determination of Vitamin (C and A). International Journal of Pharmaceutical Sciences and Research, 9(8), 3373-3377. http://dx.doi.org/10.13040/IJPSR.0975-8232.9(8).3373-77
Sharma, H., Sapkota, H. P., Khanal, A., Dhakal, O., & Gurung, R. (2019). A Comparative Analysis of Vitamin-C Concentration in Commercial Fruit Juices and Fresh Fruits of Nepal With Effect of Temperature. Indicator, 11. 46-51. https://journals.innovareacademics.in/index.php/ijpps/article/view/33408/20503
Tayar, M., Haroon, M., & Moazeen, L. (2023). Using and Validation of a New Spectrophotometric Method for Determination of Vitamin C in Pharmaceutical samples. Research Journal of Pharmacy and Technology, 16(4), 1731-1737. https://doi.org/10.52711/0974-360X.2023.00285
Timoshnikov, V. A., Kobzeva, T. V., Polyakov, N. E., & Kontoghiorghes, G. J. (2020). Redox Interactions of Vitamin C and Iron: Inhibition of the Pro-Oxidant Activity by Deferiprone. International Journal of Molecular Sciences, 21(11), 3967. https://doi.org/10.3390/ijms21113967
Yuliati, N., & Kurniawati, E. (2017). Analisis Kadar Vitamin C dan Fruktosa pada Buah Mangga (Mangifera Indica L.) Varietas Podang Urang dan Podang Lumut Metode Spektrofotometri Uv-Vis. Jurnal Wiyata: Penelitian Sains dan Kesehatan, 4(1), 49-57. http://dx.doi.org/10.56710/wiyata.v4i1.143
Aliansa, T., Munir, M. A., Aprilia, V., & Emelda, E. (2023). The Determination of Vitamin C in Guava (Myrtaceae Species) Using Spectrophotometric Approach. Asian Journal of Analytical Chemistry, 1(1), 6-11. https://doi.org/10.53866/ajac.v1i1.268
Astiti, N. P. A., & Yulihastuti, D. A. (2018). Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata). Journal of Advances in Tropical Biodiversity and EnvironmentalSciences. 1(2), 33-35. https://simdos.unud.ac.id/uploads/file_penelitian_1_dir/33aac52642bc92b6501b7d00ad12d7d6.pdf
Badriyah, L., & Manggara, A. B. (2017). Penetapan Kadar Vitamin C pada Cabai Merah (Capsicum Annum L.) Menggunakan Metode Spektrofotometri UV-VIS. Jurnal Wiyata: Penelitian Sains dan Kesehatan, 2(1), 25-28. http://dx.doi.org/10.56710/wiyata.v2i1.31
Baits, M., Jahri, R. M., Suhaenah, A., Aminah, A., Naid, T., & Fawwaz, M. (2022). The Effect of Heating on Ascorbic Acid Levels of Brassica Oleracea L. Determined by UV-Visible Spectrophotometry. Pharmaceutical Reports, 1(1). https://doi.org/10.33096/pharm%20rep.v1i1.156
Bartzatt, R., & Wol, T. (2014). Detection and Assay of Vitamin B-2 (Riboflavin) in Alkaline Borate Buffer with UV/Visible Spectrophotometry. International scholarly research notices, 2014, 453085. https://doi.org/10.1155/2014/453085
Bi, H., Fernandes, A. C., Cardoso, S., & Freitas, P. (2016). Interference-Blind Microfluidic Sensor for Ascorbic acid Determination by UV/vis Spectroscopy. Sensors and Actuators B: Chemical, 224, 668-675. https://core.ac.uk/download/pdf/43253003.pdf
Carr, A. C., & Maggini, S. (2017). Vitamin C and Immune Function. Nutrients, 9(11), 1211. https://doi.org/10.3390/nu9111211
Chandra, B., & Putri, W. D. (2019). Penetapan Kadar Vitamin C Dan B1 Pada Buah Naga Merah (Hylocereus Lemairel (Hook.) Britton & Rose) dengan Metode Spektrofotometri Uv-Vis. Jurnal Farmasi Higea, 11(1), 62-74. http://dx.doi.org/10.52689/higea.v11i1.215
Devolli, A., Stafasani, M., Shahinasi, E., Dara, F., & Hamiti, H. (2021). Determination of Vitamin C Content in Commercial Fruit Juices by Volumetric and Spectrophotometric Methods. Journal of Hygienic Engineering & Design, 34. https://www.semanticscholar.org/paper/Determination-Of-Vitamin-C-Content-In-Commercial-By-Devolli-Stafasani/88cbe990659f99a336bb764d05158d1c3ad98bf0
Dewi, A. P. (2018). Penetapan Kadar Vitamin C dengan Spektrofotometri UV-Vis Pada Berbagai Variasi Buah Tomat. JOPS (Journal Of Pharmacy and Science), 2(1), 9-13. http://dx.doi.org/10.36341/jops.v2i1.1015
Febrianti, A., Aina, G. Q., & Farpina, E. (2022). Determination of Vitamin C and ß-Carotene Levels in Several Types of Chili (Capsicum sp) Using UV-Vis Spectrophotometry Method. Formosa Journal of Science and Technology, 1(8), 1129-1142. https://doi.org/10.55927/fjst.v1i8.1949
Fitriana, Y. A. N., & Fitri, A. S. (2020). Analisis Kadar Vitamin C pada Buah Jeruk Menggunakan Metode Titrasi Iodometri. Sainteks, 17(1), 27-32. http://dx.doi.org/10.30595/sainteks.v17i1.8530
Iskandar, S. R., Yuniarto, P. F., & Sulistyowati, Y. (2023). Analysis Of Vitamin C Levels Of Curry Leaf Ethanol Extract (Murraya Koenigii L. Spreng) By Uv-Vis Spectrophotometry. Al Makki Health Informatics Journal, 1(2), 50-59. https://doi.org/10.57185/hij.v1i2.7
Jutkus, R. A., Li, N., Taylor, L. S., & Mauer, L. J. (2015). Effect of Temperature and Initial Moisture Content on the Chemical Stability and Color Change of Various Forms of Vitamin C. International Journal of Food Properties, 18(4), 862-879. https://doi.org/10.1080/10942912.2013.805770
Khasanah, N. U., & Riyanto, R. (2023). Analysis of Ascorbic Acid Content in Pineapples and Melons Using the Uv-Vis Spectrophotometry Method. Indonesian Journal of Applied Science and Engineering, 1(1), 11-17. https://e-journal.ptti.info/index.php/ijase/article/view/52/60
Lavilla, I., Romero, V., Costas, P., & Bendicho, C. (2023). Kinetic Spectrophotometric Assay for the Determination of Vitamin C in Cosmetics Following Ultrasound-Assisted Emulsification. Analytical Methods, 15(7), 951-958. https://doi.org/10.1039/D2AY01795D
Pakaya, D. (2014). Peranan Vitamin C pada Kulit. Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan, 1(2), 45-54. https://123dok.com/document/zlnj6j6q-peranan-vitamin-pakaya-tadulako-kedokteran-fakultas-kedokteran-kesehatan.html
Passos, M. L., & Saraiva, M. L. M. (2019). Detection in UV-Visible Spectrophotometry: Detectors, Detection Systems, and Detection Strategies. Measurement, 135, 896-904. https://ui.adsabs.harvard.edu/link_gateway/2019Meas..135..896P/doi:10.1016/j.measurement.2018.12.045
Putri, M. P. (2023). Variation of Processed Fresh Pineapple on Vitamin C Content Using UV-VIS Spectrophotometry. Journal of Natural Sciences and Learning, 2(1), 29-33. https://journalng.uwks.ac.id/jnsl/article/view/59
Ramírez-Cahero, H. F., & Valdivia-López, M. A. (2018). Effect of Gamma Radiation on Sugars and Vitamin C: Radiolytic Pathways. Food chemistry, 245, 1131–1140. https://doi.org/10.1016/j.foodchem.2017.11.057
Rashid, M. U., Bhuiyan, M. K. H., & Quayum, M. E. (2013). Synthesis of SILVER Nano Particles (Ag-NPs) and Their Uses for Quantitative Analysis of Vitamin C Tablets. Dhaka University Journal of Pharmaceutical Sciences, 12(1), 29-33. http://dx.doi.org/10.3329/dujps.v12i1.16297
Riscahyani, N. M. ., Ekawati, E. R., & Ngibad, K. (2019). Identification of Ascorbic Acid Content in Carica Papaya l. Using Iodimetry and Uv-Vis Spectrophotometry. Indonesian Journal of Medical Laboratory Science and Technology, 1(2), 58–64. https://doi.org/10.33086/ijmlst.v1i2.1291
Rosmainar, L., Ningsih, W., Ayu, N. P., & Nanda, H. (2018). Penentuan Kadar Vitamin C Beberapa Jenis Cabai (Capsicum SP.) dengan Spektrofotometri UV-VIS. Jurnal Kimia Riset, 3(1), 1-5. https://doi.org/10.20473/jkr.v3i1.8874
Saeed, A. M., Al-kadumi, A. S., & Ali, N. J. M. (2018). Colorimetric Determination of Antioxidant Vitamins E and C. Pakistan Journal of Biotechnology, 15(2), 451-457. https://pjbt.org/index.php/pjbt/article/view/416
Saeed, A. M., Hamzah, M. J., & Ali, N. J. M. (2018). Sensitive Spectrophotometric Method for Determination of Vitamin (C and A). International Journal of Pharmaceutical Sciences and Research, 9(8), 3373-3377. http://dx.doi.org/10.13040/IJPSR.0975-8232.9(8).3373-77
Sharma, H., Sapkota, H. P., Khanal, A., Dhakal, O., & Gurung, R. (2019). A Comparative Analysis of Vitamin-C Concentration in Commercial Fruit Juices and Fresh Fruits of Nepal With Effect of Temperature. Indicator, 11. 46-51. https://journals.innovareacademics.in/index.php/ijpps/article/view/33408/20503
Tayar, M., Haroon, M., & Moazeen, L. (2023). Using and Validation of a New Spectrophotometric Method for Determination of Vitamin C in Pharmaceutical samples. Research Journal of Pharmacy and Technology, 16(4), 1731-1737. https://doi.org/10.52711/0974-360X.2023.00285
Timoshnikov, V. A., Kobzeva, T. V., Polyakov, N. E., & Kontoghiorghes, G. J. (2020). Redox Interactions of Vitamin C and Iron: Inhibition of the Pro-Oxidant Activity by Deferiprone. International Journal of Molecular Sciences, 21(11), 3967. https://doi.org/10.3390/ijms21113967
Yuliati, N., & Kurniawati, E. (2017). Analisis Kadar Vitamin C dan Fruktosa pada Buah Mangga (Mangifera Indica L.) Varietas Podang Urang dan Podang Lumut Metode Spektrofotometri Uv-Vis. Jurnal Wiyata: Penelitian Sains dan Kesehatan, 4(1), 49-57. http://dx.doi.org/10.56710/wiyata.v4i1.143
Published
2024-05-18
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Articles
Copyright (c) 2024 Zafira Fatiha Nurulhadi, Anita Anita, Helsen Helsen, Rizky Marsada Ukur Ujung, Ermi Abriyani
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