Uji Aktivitas Antibakteri Ekstrak Etanol Daun Matoa (Pometia Pinnata J. R & G.Forst.) terhadap Pertumbuhan Staphylococcus Aureus dan Escherichia Coli
Abstract
This study aims to determine the antibacterial activity, effective concentration, and secondary metabolites in the ethanol extract of matoa left against the test bacteria Staphylococcus aureus and Escherichia coli. The research method extracts matoa leaf samples by maceration using 70% ethanol solvent, testing the antibacterial activity using the paper disk diffusion method. The results of the antibacterial activity test were then analyzed using the One way ANOVA method. The results showed that the diameter of the inhibition zone of the ethanol extract of matoa leaves on the growth of Staphylococcus aureus appeared to have a clear area at a concentration of 1%, 1.5%, and 2%, respectively, of 12.88 mm, 13.15 mm, 11.36 mm. In comparison, the results of measuring the diameter of the inhibition zone of the ethanol extract matoa leaf on the growth of Escherichia coli bacteria were seen to have a clear area at a concentration of 1%, 1.5%, and 2% of 9.5 mm, 10.23 mm, 12.1 mm respectively. This activity is thought to be due to the effects of alkaloid compounds, flavonoids, tannins, saponins, and steroids in the ethanol extract of matoa leaves. Based on the ANOVA test, a significance value of 0.000 <0.05 was obtained, so the results were significant. In conclusion, there are substantial differences in the use of various concentrations of matoa leaf extract on the growth of Staphylococcus aureus and Escherichia coli bacteria.
Keywords: Antibacterial Activity, Matoa Leaves, Secondary Metabolites
References
Fajrina, A., Bakhtra, D. D. A., & Mawarni, A. E. (2020). Isolasi dan Uji Aktivitas Antimikroba Ekstrak Etil Asetat Jamur Endofit dari Daun Matoa (Pometia Pinnata). Jurnal Farmasi Higea, 12(1), 81–89. http://jurnalfarmasihigea.org/index.php/higea/article/view/267
Hamidah, N. M., Rianingsih, L., & Romadhon, R. (2019). Aktivitas Antibakteri Isolat Bakteri Asam Laktat dari Peda dengan Jenis Ikan Berbeda terhadap E. Coli dan S. aureus. Jurnal Ilmu dan Teknologi Perikanan, 1(2), 11–20. https://ejournal2.undip.ac.id/index.php/jitpi/article/view/6742/3551
Hehakaya, M. O., Edy, H. J., & Siampa, J. P. (2022). Formulation and Antioxidant Activity Test of Body Scrub Matoa ’ s Leaves (Pometia Pinnata). Pharmacon, 11(4), 1778-1785. https://ejournal.unsrat.ac.id/v3/index.php/pharmacon/article/download/42148
Marfu’ah, N., Luthfiana, S., & Ichwanuddin, I. (2021). Uji Potensi Antibakteri Staphylococcus Aureus dari Ekstrak Etanol Daun Sirih Hijau (Piper Betle L.). Pharmaceutical Journal of Islamic Pharmacy, 5(2), 1–10. https://ejournal.unida.gontor.ac.id/index.php/pharmasipha/issue/archive
Oktaviani, A. R., Takwiman, A., Santoso, D. A. T., Hanaratri, E. O., Damayanti, E., Maghfiroh, L., & Yuda, A. (2020). Pengetahuan dan Pemilihan Obat Tradisional Oleh Ibu-Ibu di Surabaya. Jurnal Farmasi Komunitas, 8(1), 1-8. https://doi.org/10.20473/jfk.v8i1.21912
Putri, R. A., Simbala, H. E. I., & Mpila, D. A. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine Americana Merr) terhadap Bakteri Staphylococcus Aureus, Escherichia Coli dan Salmonella Typhi. Pharmacon, 9(4), 525-532. https://doi.org/10.35799/pha.9.2020.31360
Rahmah, W., Hamzah, H., Hajar, S., Ressandy, S. S., & Putri, E. M. (2021). Potential of Matoa Fruit Extract (Pometia Pinnata) As Antioxidant Source. Jurnal Farmasi Sains dan Praktis, 7(1), 59–66. https://doi.org/10.31603/pharmacy.v7i1.4240
Rossalinda, R., Wijayanti, F., & Iskandar, D. (2021). Effectiveness of Matoa Leaf (Pometia pinnata) Extract as an Antibacterial Staphylococcus Epidermidis. Stannum : Jurnal Sains dan Terapan Kimia, 3(1), 1–8. https://doi.org/10.33019/jstk.v3i1.2133
Septiyawati, F., Massinai, A., Haris, A., & Mursyid, M. (2020). Potensi Antibakteri Staphylococcus Aureus dan Escherichia Coli dari Ekstrak Kasar Bakteri Asosiasi Karang Batu yang Terinfeksi Penyakit Brown Band (BRB). Bioma, 2(2), 9–17. https://ojs.unsulbar.ac.id/index.php/bioma/article/view/855
Sidoretno, W. M., & Gustari, M. (2021). Aktivitas Antijamur Ekstrak Etanol Daun Matoa (Pometia Pinnata J.R. & G.Forst) terhadap Pertumbuhan Jamur Trichophyton Mentagrophytes. Photon: Jurnal Sain dan Kesehatan, 11(2), 137-148. https://doi.org/10.37859/jp.v11i2.2705
Sutomo, S., Hasanah, N., Arnida, A., & Sriyono, A. (2021). Standardisasi Simplisia dan Ekstrak Daun Matoa (Pometia Pinnata J.R Forst & G. Forst) Asal Kalimantan Selatan. Jurnal Pharmascience, 8(1), 101-110. https://doi.org/10.20527/jps.v8i1.10275
Tahalele, E., & Sutriningsih, S. (2018). Formulasi Sediaan Kosmetik Krim dari Ekstrak Daun Matoa (Pometia Pinnata) dan Uji Aktivitas Antioksidan. Indonesia Natural Research Pharmaceutical Journal, 3(2), 44–55. https://doi.org/10.52447/inspj.v3i2.1916
Trisia, A., Philyria, R., & Toemon, A. N. (2018). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kalanduyung (Guazuma Ulmifolia Lam.) terhadap Pertumbuhan Staphylococcus Aureus dengan Metode Difusi Cakram (Kirby-Bauer). Anterior Jurnal, 17(2), 136–143. https://doi.org/10.33084/anterior.v17i2.12
Wulandari, L., Nugraha, A. S., & Himmah, U. A. (2021). Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Matoa (Pometia pinnata J.R. Forst. & G. Forst.) secara In Vitro. Jurnal Kefarmasian Indonesia, 11(2), 132–141. https://doi.org/10.22435/jki.v11i2.3196
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