Efek Daun Etlingera hemisphaerica pada Perbaikan Skoring Spermatogenesis Tikus Putih (Rattus norvegicus) yang Diinduksi HgCl2
Abstract
This study aims to determine the effect of administration of Etlingera hemispharica leaf extract on white rats (Rattus norvegicus) induced by mercury chloride as seen from the histopathological description of the testes. The research method used is experimental post-test-only control group design. The extract was administered for seven days by gavage. Testicular organ harvesting was carried out on the 9th day after the rats were euthanized and continued with making testicular preparations. Testicular damage was assessed using the Johnsen score. Data were analyzed using non-parametric statistical tests. The results showed that the test groups had a p-value of 0.000. The histopathological description of the testes induced by HgCl2 had a p-value of 0.005 and the administration of E. hemisphaerica leaf extract in HgCl2-induced mice showed a p-value of 0.008. In conclusion, administration of E. hemisphaerica leaf extract to R. norvegicus induced by mercury chloride improved spermatogenesis on the histopathological features of the R. norvegicus testes.
Keywords: Etlingera hemispharica, HgCl2, Johnsen Score, Rattus norvegicus
References
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Monsees, T. K., Franz, M., Gebhardt, S., Winterstein, U., Schill, W. B., & Hayatpour, J. (2000). Sertoli Cells as a Target for Reproductive Hazards. Andrologia, 32(4–5), 239–246. https://doi.org/10.1046/j.1439-0272.2000.00391.x
Necib, Y., Bahi, A., Zerizer, S., Abdennour, C., & Boulakoud, M. S. (2013). Effect of Virgin Olive Oil (Olea europea. L) on Kidney Function Impairment and Oxidative Stress Induced by Mercuric Chloride in Rats. American Journal of Biochemistry and Biotechnology, 9(4), 415–422. https://doi.org/10.3844/ajbbsp.2013.415.422
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An Overview. Journal of Nutritional Science, 5, 1–3. https://doi.org/10.1017/jns.2016.41
Nijveldt, R. J., van Nood, E., van Hoorn, D. E., Boelens, P. G., van Norren, K., & van Leeuwen, P. A. (2001). Flavonoids a Review of Probable Mechanisms of Action. The American Journal of Clinical Nutrition, 74(4), 418–425. https://doi.org/10.1093/ajcn/74.4.418
Ruyani, A., Putri, R. Z. E., Jundara, P., Gresinta, E., Ansori, I., & Sundaryono, A. (2018). Protective Effect of Leaf Ethanolic Extract Etlingera hemisphaerica Blume Against Mercuric Chloride Toxicity in Blood of Mice. Journal of Dietary Supplements, 16(1), 51–65. https://doi.org/10.1080/19390211.2018.1429516
Sasikumar, V. (2015). Protective Effect of Alkaloids from Amaranthus viridis Linn. Against Hydrogen Peroxide Induced Oxidative Damage in Human Erythrocytes (RBC). International Journal of Clinical Endocrinology and Metabolism, 1(1), 49–53. https://doi.org/10.17352/ijcem.000011
Badan Pengawas Obat dan Makanan. (2016). Merkuri pada Penambang Emas. http://ik.pom.go.id/v2016/artikel/Merkuri%20Pada%20Penambangan%20Emas.pdf
Bando, I., Sánchez Reus, M. I., Andrés, D., & Cascales, M. (2005). Endogenous Antioxidant Defence System in Rat Liver Following Mercury Chloride Oral Intoxication. Journal of Biochemical and Molecular Toxicology, 19(3), 154–161. https://doi.org/10.1002/jbt.20067
Bas, H., & Kalender, S. (2016). Antioxidant Status, Lipid Peroxidation and Testis-Histoarchitecture Induced by Lead Nitrate and Mercury Chloride in Male Rats. Brazilian Archives of Biology and Technology, 59, 1–9. https://doi.org/10.1590/1678-4324-2016160151
Boujbiha, M. A., Hamden, K., Guermazi, F., Bouslama, A., Omezzine, A., Kammoun, A., & Feki, A. E. (2009). Testicular Toxicity in Mercuric Chloride Treated Rats: Association with Oxidative Stress. Reproductive Toxicology, 28(1), 81–89. https://doi.org/10.1016/j.reprotox.2009.03.011
Broussard, L. A., Hammett-stabler, C. A., & Winecker, Ruth Ropero-Miller, J. D. (2002). The Toxicology of Mercury. Laboratory Medicine, 33(8), 614-625. https://doi.org/10.1309/5HY1-V3NE-2LFL-P9MT
El-desoky, G. E., Bashandy, S. A., Alhazza, I. M., Al-othman, Z. A., Aboul-soud, M. A. M., & Yusuf, K. (2013). Improvement of Mercuric Chloride-Induced Testis Injuries and Sperm Quality Deteriorations by Spirulina platensis in Rats. PLoS One, 8(3), 1–9. https://doi.org/10.1371/journal.pone.0059177
Farina, M., Brandão, R., De Lara, F. S., Pagliosa, L. B., Soares, F. A., Souza, D. O., & Rocha, J. B. T. (2003). Profile of Nonprotein Thiols, Lipid Peroxidation and δ-aminolevulinate Dehydratase Activity in Mouse Kidney and Liver in Response to Acute Exposure to Mercuric Chloride and Sodium Selenite. Toxicology, 184(2–3), 179–187. https://doi.org/10.1016/S0300-483X(02)00576-0
Franco, J. L., Posser, T., Missau, F., Pizzolatti, M. G., Santos, A. R. S., Souza, D. O., Aschner, M., Rocha, J. B. T., Dafre, A. L., & Farina, M. (2010). Structure-Activity Relationship of Flavonoids Derived from Medicinal Plants in Preventing Methylmercury-Induced Mitochondrial Dysfunction. Environmental Toxicology and Pharmacology, 30(3), 272–278. https://doi.org/10.1016/j.etap.2010.07.003
Jackie, T., Haleagrahara, N., & Chakravarthi, S. (2011). Antioxidant Effects of Etlingera Elatior Flower Extract Against Lead Acetate-Induced Perturbations in Free Radical Scavenging Enzymes and Lipid Peroxidation in Rats. BMC Research Notes, 4, 1-7. https://doi.org/10.1186/1756-0500-4-67
Ježek, P., & Hlavatá, L. (2005). Mitochondria in Homeostasis of Reactive Oxygen Species in Cell, Tissues, and Organism. International Journal of Biochemistry and Cell Biology, 37(12), 2478–2503. https://doi.org/10.1016/j.biocel.2005.05.013
Kasote, D. M., Katyare, S. S., Hegde, M. V., & Bae, H. (2015). Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. International Journal of Biological Sciences, 11(8), 982–991. https://doi.org/10.7150/ijbs.12096
Khan, A. T., Atkinson, A., Graham, T. C., Thompson, S. J., Ali, S., & Shireen, K. F. (2004). Effects of Inorganic Mercury on Reproductive Performance of Mice. Food and Chemical Toxicology, 42(4), 571–577. https://doi.org/10.1016/j.fct.2003.10.018
Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids : An Overview. The Scientific World Journal, 2013, 1-16. https://doi.org/10.1155/2013/162750
Martinez, C. S., Escobar, A. G., Torres, J. G. D., Brum, D. S., Santos, F. W., Alonso, M. J., Salaices, M., Vassallo, D. V., Peçanha, F. M., Leivas, F. G., & Wiggers, G. A. (2014). Chronic Exposure to Low Doses of Mercury Impairs Sperm Quality and Induces Oxidative Stress in Rats. Journal of Toxicology and Environmental Health, Part A, 77(1–3), 143–154. https://doi.org/10.1080/15287394.2014.867202
Monsees, T. K., Franz, M., Gebhardt, S., Winterstein, U., Schill, W. B., & Hayatpour, J. (2000). Sertoli Cells as a Target for Reproductive Hazards. Andrologia, 32(4–5), 239–246. https://doi.org/10.1046/j.1439-0272.2000.00391.x
Necib, Y., Bahi, A., Zerizer, S., Abdennour, C., & Boulakoud, M. S. (2013). Effect of Virgin Olive Oil (Olea europea. L) on Kidney Function Impairment and Oxidative Stress Induced by Mercuric Chloride in Rats. American Journal of Biochemistry and Biotechnology, 9(4), 415–422. https://doi.org/10.3844/ajbbsp.2013.415.422
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An Overview. Journal of Nutritional Science, 5, 1–3. https://doi.org/10.1017/jns.2016.41
Nijveldt, R. J., van Nood, E., van Hoorn, D. E., Boelens, P. G., van Norren, K., & van Leeuwen, P. A. (2001). Flavonoids a Review of Probable Mechanisms of Action. The American Journal of Clinical Nutrition, 74(4), 418–425. https://doi.org/10.1093/ajcn/74.4.418
Ruyani, A., Putri, R. Z. E., Jundara, P., Gresinta, E., Ansori, I., & Sundaryono, A. (2018). Protective Effect of Leaf Ethanolic Extract Etlingera hemisphaerica Blume Against Mercuric Chloride Toxicity in Blood of Mice. Journal of Dietary Supplements, 16(1), 51–65. https://doi.org/10.1080/19390211.2018.1429516
Sasikumar, V. (2015). Protective Effect of Alkaloids from Amaranthus viridis Linn. Against Hydrogen Peroxide Induced Oxidative Damage in Human Erythrocytes (RBC). International Journal of Clinical Endocrinology and Metabolism, 1(1), 49–53. https://doi.org/10.17352/ijcem.000011
Published
2021-10-28
Section
Articles
