Histopatologi Testis pada Tikus Putih (Rattus norvegicus) yang Diberikan Minyak Jelantah dan Infusa Teh Hitam (Camellia sinensis)

  • Gina Fendiati Putri Universitas Bengkulu
  • Liya Agustin Umar Universitas Bengkulu
  • Elvira Yunita Universitas Bengkulu

Abstract

This study aimed to analyze the effect of infusion of black tea (Camellia sinensis) on the histopathological features of the testes in white rats (Rattus norvegicus) that had been induced with used cooking oil. The design of this research is an experimental post-test only control group design. The research subjects used 24 rats which were divided into six groups with six weeks of treatment. The testicular condition was assessed using the Johnsen Score criteria. The results of this study indicate that there are differences in testicular damage scores between treatment groups. The results of the histopathological score analysis showed that the group that was given only used cooking oil had a higher level of damage than the group that was only given black tea. The group that was given cooking oil and black tea one hour later affected testicular damage in rats with p significance values ​​of 0.018 and 0.018. In conclusion, giving black tea infusion at a dose of 0.50 g/200 gram BW and 0.75 gr/200 gram BW was able to prevent damage due to oil induction with 12x heating in the testes of Rattus norvegicus rats.

Keywords: Camellia sinensis, Histopathology, Cooking Oil, Rat Testes

References

Ananto, A. S., Wulan, A. J., Kedokteran, F., Lampung, U., Anatomi, B., Kedokteran, F., Lampung, U., Kedokteran, B. P., Kedokteran, F., & Lampung, U. (2017). Pengaruh Pemberian Minyak Jelantah terhadap Perbedaan Rerata Kerusakan Gambaran Histologi Jaringan Usus Halus Tikus Jantan (Rattus norvegicus) Galur Sprague dawley. Medula, 7(5), 187–193. http://juke.kedokteran.unila.ac.id/index.php/medula/article/view/2006/pdf

Asadi, N., Bahmani, M., Kheradmand, A., & Rafieian-Kopaei, M. (2017). The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving It: A Review. Journal of Clinical and Diagnostic Research, 11(5), 1–5. https://doi.org/10.7860/JCDR/2017/23927.9886

Adwas, A. A., Elsayed, A. S. I., Azab, A. A., & Quwaydir, F. A. (2019). Oxidative Stress and Antioxidant Mechanisms in Human Body. Journal of Applied Biotechnology & Bioengineering, 6(1), 43–47. https://doi.org/10.15406/jabb.2019.06.00173

Azizi, P., & Mehranjani, M. S. (2019). The Effect of Green Tea Extract on the Sperm Parameters and Histological Changes of Testis in Rats Exposed to Para-Nonylphenol. International Journal of Reproductive BioMedicine, 17(10), 717–726. https://doi.org/10.18502/ijrm.v17i10.5290

Bernatoniene, J., & Kopustinskiene, D. M. (2018). The Role of Catechins in Cellular Responses to Oxidative Stress. Molecules, 23(4), 1–11. https://doi.org/10.3390/molecules23040965

Chang, M. Y., Lin, Y. Y., Chang, Y. C., Huang, W. Y., Lin, W. S., Chen, C. Y., Huang, S. L., & Lin, Y. S. (2020). Effects of Infusion and Storage on Antioxidant Activity and Total Phenolic Content of Black Tea. Applied Sciences (Switzerland), 10(8), 1-10. https://doi.org/10.3390/APP10082685

Fanani, N., & Ningsih, E. (2019). Analisis Kualitas Minyak Goreng Habis Pakai yang Digunakan oleh Pedagang Penyetan di Daerah Rungkut Surabaya Ditinjau dari Kadar Air dan Kadar Asam Lemak Bebas (ALB). Jurnal IPTEK, 22(2), 59–66. https://doi.org/10.31284/j.iptek.2018.v22i2.436

Kamiński, P., Baszyński, J., Jerzak, I., Kavanagh, B. P., Nowacka-Chiari, E., Polanin, M., Szymański, M., Woźniak, A., & Kozera, W. (2020). External and Genetic Conditions Determining Male Infertility. International Journal of Molecular Sciences, 21(15), 1–27. https://doi.org/10.3390/ijms21155274

Khor, Y. P., Hew, K. S., Abas, F., Lai, O. M., Cheong, L. Z., Nehdi, I. A., Sbihi, H. M., Gewik, M. M., & Tan, C. P. (2019). Oxidation and Polymerization of Triacylglycerols: In-Depth Investigations towards the Impact of Heating Profiles. Foods, 8(10), 1–15. https://doi.org/10.3390/foods8100475

Nguyen-Powanda, P., & Robaire, B. (2020). Oxidative Stress and Reproductive Function in the Aging Male. Biology, 9(9), 1–15. https://doi.org/10.3390/biology9090282

Opuwari, C. S., & Monsees, T. K. (2020). In Vivo Effects of Black Tea on the Male Rat Reproductive System and Functions of the Kidney and Liver. Andrologia, 52(4), 1–10. https://doi.org/10.1111/and.13552

Panjiasih Susmiarsih, T., Kenconoviyati, K., & Kuslestari, K. (2018). Potensi Ekstrak Daun Teh Hijau terhadap Morfologi dan Motilitas Spermatozoa Tikus Putih (Rattus norvegicus) setelah Paparan Asap Rokok. Majalah Kesehatan Pharmamedika, 10(1), 1-7. https://doi.org/10.33476/mkp.v10i1.682

Sayiner, S., Gülmez, N., Sabit, Z., & Gülmez, M. (2019). Effects of Deep-Frying Sunflower Oil on Sperm Parameters in a Mouse Model: Do Probiotics Have a Protective Effect? Kafkas Universitesi Veteriner Fakultesi Dergisi, 25(6), 857–863. https://doi.org/10.9775/kvfd.2019.22063

Sekaran, K., & Semarang, G. (2018). Pengolahan Minyak Goreng Bekas (Jelantah) sebagai Pengganti Bahan Bakar Minyak Tanah (Biofuel) bagi Pedagang Gorengan di Sekitar FMIPA UNNES. Rekayasa, 15(2), 89–95. https://doi.org/10.15294/rekayasa.v15i2.12588

Tong, T., Liu, Y. J., Kang, J., Zhang, C. M., & Kang, S. G. (2019). Antioxidant Activity and Main Chemical Components of a Novel Fermented Tea. Molecules, 24(16), 1–14. https://doi.org/10.3390/molecules24162917

Treuting, P. M., Dintzis, S. M., & Montine, K. S., 2017. Comparative Anatomy dan Histology: A Mouse, Rat, dan Human Atlas. 2nd edn. USA: Academic Press
Published
2021-06-04
How to Cite
Putri, G., Umar, L., & Yunita, E. (2021). Histopatologi Testis pada Tikus Putih (Rattus norvegicus) yang Diberikan Minyak Jelantah dan Infusa Teh Hitam (Camellia sinensis). BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 4(1), 48-61. https://doi.org/https://doi.org/10.31539/bioedusains.v4i1.2171
Abstract viewed = 1 times
pdf downloaded = 1 times