Efek Ekstrak Jintan Hitam (Nigella sativa L.) terhadap Gambaran Histopatologi Hepar Tikus Putih (Rattus norvegicus) yang Diinduksi Isoniazid
Abstract
This study aimed to examine the effect of giving black cumin extract to changes in the histopathological picture of the liver of white rats induced by isoniazid. The method used is experimental with posttest only control group design pattern. The research subjects used 25 Rattus norvegicus, which were divided into negative control group (P1), positive control (P2) isoniazid orally with a dose of 5.4 - 6.75 mg/day for six weeks, treatment 1 (P3), treatment 2 (P4 ), and treatment 3 (P5) which was given isoniazid at a dose of 5.4 - 6.75 mg/day orally and followed by oral black cumin with stratified amounts of 2000, 2500 and 3000 mg/kgBW. The results showed that there was a significant increase in liver cell damage after administration of isoniazid (p < 0.05) and liver cell recovery after administration of black cumin extract for 42 days (p < 0.05). In conclusion, the administration of black cumin extract can restore isoniazid-induced liver cell damage.
Keywords: Liver Histopathology, Isoniazid, Black Cumin
References
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World Health Organization (WHO). (2017). Global Tuberculosis Report 2017. https://www.who.int/tb/publications/global_report/gtbr2017_main_text.pdf
Ahmad, M. F., Ahmad, F. A., Ashraf, S. A., Saad, H. H., Wahab, S., Khan, M. I., Ali, M., Mohan, S., Hakeem, K. R., & Athar, M. T. (2020). An Updated Knowledge of Black Seed (Nigella sativa Linn): Review of Phytochemical Constituents and Pharmacological Properties. Journal of herbal medicine. https://doi.org/10.1016/j.hermed.2020.100404
Farooqui, Z., Ahmed, F., Rizwan, S., Shahid, F., Khan, A. A., & Khan, F. (2017). Protective Effect of Nigella sativa Oil on Cisplatin Induced Nephrotoxicity and Oxidative Damage in Rat Kidney. Journal of Biomedicine & Pharmacotherapy, 85, 7–15. https://doi.org/10.1016/j.biopha.2016.11.110
Goyal, S. N., Prajapati, C. P., Gore, P. R., Patil, C. R., Mahajan, U. B., Sharma, C., Talla, S. P., & Ojha, S. K. (2017). Therapeutic Potential and Pharmaceutical Development of Thymoquinone: A Multitargeted Molecule of Natural Origin. Frontiers in pharmacology, 8, 1-19. https://doi.org/10.3389/fphar.2017.00656
Kastien-Hilka, T., Rosenkranz, B., Schwenkglenks, M., Bennett, B. M., & Sinanovic, E. (2017). Association between Health-Related Quality of Life and Medication Adherence in Pulmonary Tuberculosis in South Africa. Frontiers in Pharmacology, 8, 1-7. https://dx.doi.org/10.3389%2Ffphar.2017.00919
Kementerian Kesehatan Republik Indonesia. (2018). Tuberkulosis (TB). https://pusdatin.kemkes.go.id/resources/download/pusdatin/infodatin/infodatin-tuberkulosis-2018.pdf
Khan, M. A. (2019). Thymoquinone, A Constituent of Prophetic Medicine-Black Seed, is A Miracle Therapeutic Molecule Against Multiple Diseases. International journal of health sciences, 13(1), 1-2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392482/pdf/IJHS-13-1.pdf
Mukhtar, H., Qureshi, A. S., Anwar, F., Mumtaz, M. W., & Marcu, M. (2019). Nigella sativa L. Seed and Seed Oil: Potential Sources of High-Value Components for Development of Functional Foods snd Nutraceuticals/Pharmaceuticals. Journal of Essential Oil Research 31(3), 171–183. https://doi.org/10.1080/10412905.2018.1562388
Niu, Y., Zhou, L., Meng, L., Chen, S., Ma, C., Liu, Z., & Kang, W. (2020). Recent Progress on Chemical Constituents and Pharmacological Effects of the Genus Nigella. Evidence-Based Complementary and Alternative Medicine, 2020, 1-15. https://doi.org/10.1155/2020/6756835
Saha, P., Talukdar, A. Das, Nath, R., Sarker, S. D., Nahar, L., Sahu, J., & Choudhury, M. D. (2019). Role of Natural Phenolics in Hepatoprotection: A Mechanistic Review and Analysis of Regulatory Network of Associated Genes. Frontiers in Pharmacology, 10. https://doi.org/10.3389/fphar.2019.00509
Salahshoor, M. R., Vahabi, A., Roshankhah, S., Darehdori, A. S., & Jalili, C. (2018). The Effects of Thymoquinone Against Morphine-induced Damages on Male Mice Liver. International Journal of Preventive Medicine, 9, 1-6. https://doi.org/10.4103/ijpvm.IJPVM_144_16
Tabassum, H., Ahmad, A., & Ahmad, I. Z. (2018). Nigella sativa L. and Its Bioactive Constituents as Hepatoprotectant: A Review. Current Pharmaceutical Biotechnology, 19(1), 43–67. https://doi.org/10.2174/1389201019666180427110007
Tavakkoli, A., Mahdian, V., Razavi, B. M., & Hosseinzadeh, H. (2017). Review on Clinical Trials of Black Seed (Nigella sativa) and Its Active Constituent, Thymoquinone. Journal of Pharmacopuncture, 20(3), 179–193. https://doi.org/10.3831/KPI.2017.20.021
Vilchèze, C., & Jacobs Jr, W. R. (2019). The Isoniazid Paradigm of Killing, Resistance, and Persistence in Mycobacterium Tuberculosis. Journal of molecular biology, 431(18), 3450–3461.
Wei, J., Li, Y., Wang, H., Pei, D., Wang, N., Di, D., & Liu, Y. (2021). Protective Effect of Lycium Barbarum Polysaccharides on Anti-Tuberculosis Drug-Induced Injury to Human Hepatocytes and Its Mechanism. Research Square, 1-13. https://doi.org/10.21203/rs.3.rs-698780/v1
World Health Organization (WHO). (2017). Global Tuberculosis Report 2017. https://www.who.int/tb/publications/global_report/gtbr2017_main_text.pdf
Published
2021-10-19
Section
Articles
