Aktivitas Antibakteri Ekstrak Etanol Daun Tapak Kuda (Ipomoea pes-caprae (L.) R. Br.) terhadap Bakteri Staphylococcus epidermidis
Abstract
This study aimed to determine the antibacterial activity of the ethanol extract of the leaves of Tapak Kuda (Ipomoea pes-caprae) against Staphylococcus epidermidis. The method used was Kirby-Bauer for the antibacterial test with various concentrations of ethanolic extract of I. pes-caprae leaf extract: 5 mg/mL, 20 mg/mL, 35 mg/mL, and 50 mg/mL, with five replications. The antibiotic clindamycin was used as a positive control, while DMSO was a negative control. The results showed that after 24 hours of incubation, the zone of inhibition of bacterial growth was demonstrated by the experimental and the positive control groups, while the negative control did not show the zone of inhibition. The diameter of the largest inhibition zone (11.40 mm) was at a 50 mg/mL test concentration, which was indicated as a strong category. In conclusion, the ethanolic extract of the leaves of I. pes-caprae can inhibit the growth of S. epidermidis at all test concentrations.
Keywords: Antibacterial, Ipomoea pes-caprae, Staphylococcus epidermidis
References
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Bragadeeswaran, S., Prabhu, K., Sophia Rani, S., Priyadharsini, S., & Vembu, N. (2010). Biomedical Application of Beach Morning Glory Ipomoea pes-caprae. International Journal of Tropical Medicine, 5(4), 81–85. https://doi.org/10.3923/ijtmed.2010.81.85
Chan, E. W. C., Baba, S., Chan, H. T., Kainuma, M., & Tangah, J. (2016). Medicinal Plants of Sandy Shores: A Short Review on Vitex trifolia L. and Ipomoea pes-caprae (L.) R. Br. Indian Journal of Natural Products and Resources, 7(2), 107–115. http://op.niscair.res.in/index.php/IJNPR/article/view/12710/863
Ginovyan, M., Petrosyan, M., & Trchounian, A. (2017). Antimicrobial Activity of Some Plant Materials Used in Armenian Traditional Medicine. BMC Complementary and Alternative Medicine, 17(50), 1–9. https://doi.org/10.1186/s12906-017-1573-y
Gonelimali, F. D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S. R. (2018). Antimicrobial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms. Frontiers in Microbiology, 9, 1-9. https://doi.org/10.3389/fmicb.2018.01639
Kiriwenno, J. V., Yunita, M., & Latuconsina, V. Z. (2021). Perbandingan Aktivitas Antibakteri Antara Ekstrak Daun Katang-Katang (Ipomoea pes-caprae L.) dan Minyak Seith terhadap Pertumbuhan Staphylococcus aureus. Majalah Farmaseutik, 17(1), 122–131. https://doi.org/10.22146/farmaseutik.v17i1.58292
Kumar, A., Paul, S., Pingalkumari, Somasundaram, S. T., & Kathiresan, K. (2014). Antibacterial and Phytochemical Assessment on Various Extracts of Ipomoea pes-caprae (L.) R. Br through FTIR and GC- MS Spectroscopic Analysis. Asian Journal of Pharmaceutical and Clinical Research, 7(3), 134–138. https://innovareacademics.in/journals/index.php/ajpcr/article/download/1188/818
Nilam, R., Jyoti, P., & Sumitra, C. (2018). Pharmacognostic and Phytochemical Studies of Ipomoea pes-caprae, An Halophyte from Gujarat. Journal of Pharmacognosy and Phytochemistry, 7(1), 11–18. http://www.phytojournal.com/archives/2018/vol7issue1/PartA/6-6-169-989.pdf
Pejin, B., Savic, A., Sokovic, M., Glamoclija, J., Ciric, A., Nikolic, M., Radotic, K., & Mojovic, M. (2014). Further in Vitro Evaluation of Antiradical and Antimicrobial Activities of Phytol. Natural Product Research, 28(6), 372–376. https://doi.org/10.1080/14786419.2013.869692
Saimima, N. A., & Manuhuttu, D. (2021). Potensi Ekstrak Daun Tapak Kuda (Ipomoea pes-caprae) Sebagai Antibakteri Patogen Pangan. Journal of Aceh Aquatic Science, 5(1), 1–19. https://doi.org/10.35308/jaas.v5i1.3573
Singh, S. P., Qureshi, A., & Hassan, W. (2021). Mechanisms of Action by Antimicrobial Agents: A Review. McGill Journal of Medicine, 19(4), 1–10. https://doi.org/10.26443/mjm.v19i1.217
Tan, J., Frey, M. P., Thiboutot, D., Layton, A., & Eady, A. (2020). Identifying the Impacts of Acne: A Delphi Survey of Patients and Linicians. Journal of Cutaneous Medicine and Surgery, 24(3), 259–266. https://doi.org/10.1177/1203475420907088
Wardhani, F. K., & Poedjirahajoe, E. (2020). Potensi Pemanfaatan Ipomoea pes-caprae (L.) R. Br. di Hutan Pantai Petanahan Kebumen. Jurnal Ilmu Kehutanan, 14(2), 145–153. https://doi.org/10.22146/jik.61398
Yang, J. H., Hwang, E. J., Moon, J., Yoon, J. Y., Kim, J. W., Choi, S., Cho, S. I., & Suh, D. H. (2021). Clinical Efficacy of Herbal Extracts in Treatment of Mild to Moderate Acne Vulgaris: An 8-Week, Double-Blinded, Randomized, Controlled Trial. Journal of Dermatological Treatment, 32(3), 297–301. https://doi.org/10.1080/09546634.2019.1657792
Zhou, J., Li, X., Chen, H., Qi, Z., Shao, S., Tang, Y., & Jiang, C. (2021). Effects and Safety of Acne Vulgaris with External Application of Herbal Medicines: A Protocol for Systematic Review and Meta Analysis. Medicine, 100(26), 1-6. https://doi.org/10.1097/MD.0000000000026408
Published
2021-11-01
Section
Articles
