Isolasi dan Karakterisasi Bakteri Asal Sumber Air Panas Non-Vulkanik
Abstract
This study aims to isolate and characterize bacteria that live in hot springs on Bangka Island. The research methods used include bacterial isolation; morphological characterization, Gram group determination and biochemical tests; identification; and thermophilic bacteria temperature resistance test. Hot water samples were obtained from 2 locations, namely Pemali Village and Permis Village. The hot water temperature range at both locations is between 38ºC to 52ºC. The results showed that 22 pure isolates were consisting of 9 isolates from Permis Village and 13 isolates from Pemali Village. In conclusion, the characterization results showed that all bacterial isolates were gram-negative bacteria with various biochemical characteristics.
Keywords: Thermophilic Bacteria, Non-Volcanic, Bangka Island
References
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York, A. (2018). Environmental Microbiology: Marine Biogeochemical Cycles in a Changing World. Nature Reviews Microbiology, 16(5), 259. https://doi.org/10.1038/nrmicro.2018.40
Ardhi, A., Sidauruk, A. N., Suraya, N., Pratiwi, N. W., Pato, U., & Saryono. (2020). Molecular Identification of Amylase-Producing Thermophilic Bacteria Isolated from Bukit Gadang Hot Spring, West Sumatra, Indonesia. Biodiversitas, 21(3), 994–1000. https://doi.org/10.13057/biodiv/d210319
Arora-Williams, K., Olesen, S. W., Scandella, B. P., Delwiche, K., Spencer, S. J., Myers, E. M., Abraham, S., Sooklal, A., & Preheim, S. P. (2018). Dynamics of Microbial Populations Mediating Biogeochemical Cycling in a Freshwater Lake. Microbiome, 6(1), 1–16. https://doi.org/10.1186/s40168-018-0556-7
Arzita, A., Syamsuardi, S., Agustien, A., & Rilda, Y. (2017). The Diversity of the Alkaline Protease Producers, Thermophilic Obligate Bacillus spp., from Sungai Tutung Hot Spring, Kerinci, Jambi, Indonesia. Journal of Pure and Applied Microbiology, 11(4), 1789–1797. https://doi.org/10.22207/JPAM.11.4.18
Attri, P., Han, J., Choi, S., Choi, E. H., Bogaerts, A., & Lee, W. (2018). CAP Modifies the Structure of a Model Protein from Thermophilic Bacteria: Mechanisms of Cap-Mediated Inactivation. Scientific Reports, 8(1), 1–10. https://doi.org/10.1038/s41598-018-28600-w
El-Gayar, K. E., Al Abboud, M. A., & Essa, A. M. M. (2017). Characterization of Thermophilic Bacteria Isolated From Two Hot Springs in Jazan, Saudi Arabia. Journal of Pure and Applied Microbiology, 11(2), 743–752. https://doi.org/10.22207/JPAM.11.2.13
Febiyanto, F. (2020). Effects of Temperature and Aeration on the Dissolved Oxygen (DO) Values in Freshwater Using Simple Water Bath Reactor: A Brief Report. Walisongo Journal of Chemistry, 3(1), 25-30. https://doi.org/10.21580/wjc.v3i1.6108
Hamuna, B., Tanjung, R. H. R., Suwito, S., Maury, H. K., & Alianto, A. (2018). Kajian Kualitas Air Laut dan Indeks Pencemaran Berdasarkan Parameter Fisika-Kimia di Perairan Distrik Depapre, Jayapura. Jurnal Ilmu Lingkungan, 16(1), 35-43. https://doi.org/10.14710/jil.16.1.35-43
Irdawati, I., Syamsuardi, S., Agustien, A., & Rilda, Y. (2018). Screening of Thermophilic Bacteria Produce Xylanase from Sapan Sungai Aro Hot Spring South Solok. IOP Conference Series: Materials Science and Engineering, 335(1), 1-12. https://doi.org/10.1088/1757-899X/335/1/012021
Lischer, K., Putra, A. B. R. D., Guslianto, B. W., Avilla, F., Sitorus, S. G., Nugraha, Y., & Sarmoko. (2020). Short Communication: The Emergence and Rise of Indigenous Thermophilic Bacteria Exploration from Hot Springs in Indonesia. Biodiversitas, 21(11), 5474–5481. https://doi.org/10.13057/biodiv/d211156
Oberbeckmann, S., Kreikemeyer, B., & Labrenz, M. (2018). Environmental Factors Support The Formation of Specific Bacterial Assemblages on Microplastics. Frontiers in microbiology, 8, 1–12. https://doi.org/10.3389/fmicb.2017.02709
Ovando-Chacon, S. L., Tacias-Pascacio, V. G., Ovando-Chacon, G. E., Rosales-Quintero, A., Rodriguez-Leon, A., Ruiz-Valdiviezo, V. M., & Servin-Martinez, A. (2020). Characterization of Thermophilic Microorganisms in The Geothermal Water Flow of El Chichón Volcano Crater Lake. Water (Switzerland), 12(8), 1-16. https://doi.org/10.3390/W12082172
Rantuboi, D. Y. P., Gunaedi, T., Simonapendi, M., & Pakpahan, N. (2018). Isolasi dan Identifikasi Bakteri Termofilik dari Sumber Air Panas di Moso Distrik Muara Tami Kota Jayapura Provinsi Papua. J Biol Papua, 10(2), 68–73. https://ejournal.uncen.ac.id/index.php/JBP/index
Sang, P., Liu, S. Q., & Yang, L. Q. (2020). New Insight into Mechanisms of Protein Adaptation to High Temperatures: A Comparative Molecular Dynamics Simulation Study of Thermophilic and Mesophilic Subtilisin-Like Serine Proteases. International Journal of Molecular Sciences, 21(9), 1-12. https://doi.org/10.3390/ijms21093128
Villanueva, L., von Meijenfeldt, F. A. B., Westbye, A. B., Yadav, S., Hopmans, E. C., Dutilh, B. E., & Damsté, J. S. S. (2021). Bridging the Membrane Lipid Divide: Bacteria of the FCB Group Superphylum Have the Potential to Synthesize Archaeal Ether Lipids. ISME Journal, 15(1), 168–182. https://doi.org/10.1038/s41396-020-00772-2
Widyaningrum, Y., & Kurniawan, W. B. (2019). Karakteristik Batuan Penyusun Sumber Mata Air Panas Desa Nyelanding Kecamatan Air Gegas Kabupaten Bangka Selatan (Sebuah Rangkuman). Seminar Nasional Penelitian dan Pengabdian pada Masyarakat, 2–5. https://journal.ubb.ac.id/index.php/snppm/article/view/1362
Wu, Z., Liu, Q., Li, Z., Cheng, W., Sun, J., Guo, Z., Li, Y., Zhou, J., Meng, D., Li, H., Lei, P., & Yin, H. (2018). Environmental Factors Shaping the Diversity of Bacterial Communities that Promote Rice Production. BMC Microbiology, 18(1), 1–11. https://doi.org/10.1186/s12866-018-1174-z
Xia, Y. L., Sun, J. H., Ai, S. M., Li, Y., Du, X., Sang, P., Yang, L. Q., Fu, Y. X., & Liu, S. Q. (2018). Insights into the Role of Electrostatics in Temperature Adaptation: A Comparative Study of Psychrophilic, Mesophilic, and Thermophilic Subtilisin-Like Serine Proteases. RSC Advances, 8(52), 29698–29713. https://doi.org/10.1039/c8ra05845h
York, A. (2018). Environmental Microbiology: Marine Biogeochemical Cycles in a Changing World. Nature Reviews Microbiology, 16(5), 259. https://doi.org/10.1038/nrmicro.2018.40
Published
2021-08-17
Section
Articles
