Efektivitas Variasi Ukuran Botol Media Tanam Terhadap Pertumbuhan Planlet Anggrek Phalaenopsis amabilis L.
Abstract
This study aims to determine the effect of variations in media bottle size on the growth of Phalaenopsis amabilis L. orchid plantlets. This study used a Completely Randomized Design (CRD) with variations in one factor, namely the size of the media bottle (P1: 250 ml, P2: 500 ml, and P3: 750 ml). Each treatment variation was repeated four times. The results of data analysis in this study used the SPSS 27 program. The data was tested for normality and homogeneity, then continued with the ANOVA (Analysis of Variance) test at the 5% level. If there is a significant influence then proceed with the Least Significant Difference (BNT) test. The results of the research showed that there was an effect of using variations in the size of the 750 ml bottle on the parameters of root length and number of roots. In conclusion, variations in the size of the planting medium bottle can influence the growth of Phalaenopsis amabilis L. orchid plantlets including root length and number of roots.
Keywords: Tissue Culture, Phalaenopsis amabilis L., Bottle Size
References
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Solihah, S. F., Supriyatna, A., & Adawiyah, A. (2021). Pengaruh konsentrasi air kelapa (Cocos nucifera L.) terhadap eksplan krisan (Chrysanthemum morifolium) kultivar “xanne agrihorti” secara in vitro. Gunung Djati Conference Series, 6, 2021. https://conference.uinsgd.ac.id/index.php/
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Surata, I. K. (2012). Pertumbuhan semai cendana (Santalum album Linn.) pada beberapa ukuran kantung plastik di daerah semiarid. Jurnal Penelitian Kehutanan Wallacea, 1(1), 13-25. https://doi.org/10.18330/jwallacea.2012.vol1iss1pp13-25
Taulabi, D., Himawati, S., Nurhangga, A., Bidara, I. S., Aprianti, R., Devy, L., & Pitono, J. (2024). Pengaruh ketinggian AB mix terhadap pertumbuhan caisim menggunakan modifikasi hidroponik sistem Wick. J. Hort. Indonesia, 15(1), 16-22. http://doi.org/10.29244/jhi.15.1.16-22
Utami, E. S. W., & Hariyanto, S. (2019). In vitro seed germination and seedling development of a rare indonesian native orchid phalaenopsis amboinensis J.J.Sm. Scientifica, 2019, Article 8105138. https://doi.org/10.1155/2019/8105138
Zahara, M. (2017). A review: Micropropagation of Phalaenopsis sp from leaf and flower stalk explants. Jurnal Natural, 17(2), 91–95. https://doi.org/10.24815/jn.v17i2.8130
Ari, H. G., Melati, M., & Aziz, S. (2016). Produksi bibit tempuyung (Sonchus arvensis L.) dengan komposisi dan volume media tumbuh yang berbeda. Jurnal Hortikultura Indonesia, 7(3), 195–203. https://doi.org/10.29244/jhi.7.3.195-203
Asra, R., Samarlina, R. A., & Silalahi, M. (2020). Hormon tumbuhan. Jakarta: UKI Press
De, L. C., & Singh, R. (2018). Organic production of cymbidium orchids. Acta Scientific Agriculture, 2(4), 1–6. https://www.researchgate.net/publication/335443737
Dewi, E. R. S., Nurgroho, A. S., & Ulfah, M. (2020). Types of Epiphytic orchids and host plants on ungaran mountain limbangan kendal central java and its potential as orchid conservation area. International Journal of Conservation Science, 11(1), 117–124. www.ijcs.uaic.ro
Elendrya, S., Sesanti, R. N., Erfa, L., Sismanto, S., & Prajaka, N. W. (2023). Pengaruh berbagai jenis dan volume media tanam terhadap pertumbuhan dan hasil melon (Cucumis melo L.) dengan sistem hidroponik. J. of Horticulture Production Technology, 1(1), 20–29. https://jurnal.polinela.ac.id/jht
Heriansyah, P. (2019). Multiplikasi embrio somatis tanaman anggrek (Dendrobium sp) dengan pemberian kinetin dan sukrosa secara in-vitro. Jurnal Ilmiah Pertanian, 15(2), 67–78. https://doi.org/10.31849/jip.v15i2.1974
Hinsley, A., De Boer, H. J., Fay, M. F., Gale, S. W., Gardiner, L. M., Gunasekara, R. S., Kumar, P., Masters, S., Metusala, D., Roberts, D. L., Veldman, S., Wong, S., & Phelps, J. (2018). A review of the trade in orchids and its implications for conservation. Botanical Journal of the Linnean Society, 186(4), 435–455. https://doi.org/10.1093/botlinnean/box083
Istiqomah, M. A., Setiari, N., & Nurchayati, Y. (2020). Pengaruh media MS dan VW terhadap pertumbuhan planlet anggrek bulan (Phalaenopsis amabilis L. . Blume). Strategi Sains, 1(1), 476–480.
Krisdianto, A., Saptiningsih, E., Nurchayati, Y., & Setiari, N. (2020). Pertumbuhan plantlet anggrek Phalaenopsis amabilis (L.) blume pada tahap subkultur dengan perlakuan jenis media dan konsentrasi pepton berbeda. Metamorfosa: Journal of Biological Sciences, 7(2), 40. https://doi.org/10.24843/metamorfosa.2020.v07.i02.p06
Meilasari, D., & Iriawati, I. (2016). Regeneration of plantlets through PLB (Protocorm-like body) formation in Phalaenopsis ‘join angle X Sogo Musadian. Journal of Mathematical and Fundamental Sciences, 48(3), 204–212. https://doi.org/10.5614/j.math.fund.sci.2016.48.3.2
Ningsih, T. I. S., Nurcahyani, E., Zulkifli, Z., & Irawan, B. (2021). Pertumbuhan planlet anggrek Cattleya sp. setelah penambahan ekstrak tomat (Solanum lycopersicum L.) pada medium vacin and went. Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 19(2), 158–165. https://doi.org/10.32528/agritrop.v19i2.6465
Rahayu, A., & Susanto, S. (2016). Pertumbuhan tanaman pamelo (Citrus maxima (Burm.) Merr.) pada berbagai komposisi dan volume media tanam. J. Hort. Indonesia, 7(1), 40–48. https://doi.org/10.29244/jhi.7.1.40-48
Rahman, A., Anugrahwati, D. R., & Zubaidi, A. (2022). Uji daya hasil beberapa genotip tanaman sorgum (Sorghum bicolor. L Moench) di lahan kering lombok utara. Jurnal Ilmiah Mahasiswa Agrokomplek, 1(2), 164–171. https://doi.org/10.29303/jima.v1i2.1448
Rodinah, R., Hardarani, N., & Ariani, H. D. (2018). Modifikasi media dan periode subkultur pada kultur jaringan pisang talas (Musa paradisiaca Var. Sapientum L.). Jurnal Hexagro, 2(2), 1–6. https://doi.org/10.36423/hexagro.v2i2.129
Sari, G. M., Ulfah, M., & Mulyaningrum, E. R. (2024). Studi banding media tanam kayu kopi dan papan pakis pada Phalaenopsis amabilis pertumbuhan vegetatif pada fase pembibitan. Jurnal, 8(1), 59–66. https://doi.org/10.22263/jbes/13077
Solihah, S. F., Supriyatna, A., & Adawiyah, A. (2021). Pengaruh konsentrasi air kelapa (Cocos nucifera L.) terhadap eksplan krisan (Chrysanthemum morifolium) kultivar “xanne agrihorti” secara in vitro. Gunung Djati Conference Series, 6, 2021. https://conference.uinsgd.ac.id/index.php/
Sulichantini, E. D., Eliyani, Saputra, A., Nazari, A. P. D., & Susylowati. (2021). Pengaruh zat pengatur tumbuh dan bahan organik terhadap pertumbuhan anggrek tebu Grammatophyllum speciosum Blume secara kultur jaringan. Jurnal AgroekoteknologiTropikaLembab, 4(1), 13–19. http://dx.doi.org/10.35941/jatl.4.1.2021.5791.%25p
Sundalangi, G., Mandang, J., & Sompotan, S. (2023). Perlakuan Air kelapa tua , dan BAP pada media MS , VW terhadap protocorm anggrek Dendrobium sp . secara kultur in vitro. Agri-SosioEkonomi Unsrat, 19(1), 571–578. https://doi.org/10.35791/agrsosek.v19i1.46754
Surata, I. K. (2012). Pertumbuhan semai cendana (Santalum album Linn.) pada beberapa ukuran kantung plastik di daerah semiarid. Jurnal Penelitian Kehutanan Wallacea, 1(1), 13-25. https://doi.org/10.18330/jwallacea.2012.vol1iss1pp13-25
Taulabi, D., Himawati, S., Nurhangga, A., Bidara, I. S., Aprianti, R., Devy, L., & Pitono, J. (2024). Pengaruh ketinggian AB mix terhadap pertumbuhan caisim menggunakan modifikasi hidroponik sistem Wick. J. Hort. Indonesia, 15(1), 16-22. http://doi.org/10.29244/jhi.15.1.16-22
Utami, E. S. W., & Hariyanto, S. (2019). In vitro seed germination and seedling development of a rare indonesian native orchid phalaenopsis amboinensis J.J.Sm. Scientifica, 2019, Article 8105138. https://doi.org/10.1155/2019/8105138
Zahara, M. (2017). A review: Micropropagation of Phalaenopsis sp from leaf and flower stalk explants. Jurnal Natural, 17(2), 91–95. https://doi.org/10.24815/jn.v17i2.8130
Published
2024-08-21
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