Pemanfaatan Hidrolisat Talas (Colocasia esculenta L.) pada Kultur Miksotrofik Mikroalga Tetraselmis chui: Pengamatan Pertumbuhan dan Kadar Lipida

  • Mohamad Agus Salim UIN Sunan Gunung Djati Bandung

Abstract

Penelitian ini bertujuan untuk mengamati potensi penggunaan hidrolisat talas (Colocasia esculenta L.) sebagai sumber karbon organik kompleks untuk menghasilkan kerapatan sel, biomasa, kadar lipida dan klorofil yang optimum dari mikroalga Tetraselmis chui pada  kondisi miksotrofik. Kultur dilaksanakan pada botol yang berisi 400 mL medium basal bold. Hidrolisat talas diberikan dalam lima konsentrasi yaitu : 0, 5, 10, 15 dan 20 g/L. Hasilnya penelitian memperlihatkan mikroalga T. chui dapat tumbuh pada kondisi miksotrofik dengan penambahan hidrolisat talas pada kisaran konsentrasi 5 sampai 20 g/L. Kerapatan sel dan berat kering maksimum  pada penambahan hidrolisat talas 10 g/L yaitu masing masing 4,23 x 105 sel/ml dan 0,57 g/L. Sedangkan berat segar dan kadar lipida maksimum dicapai pada penambahan hidrolisat talas 15 g/L yaitu 4,85 g/L dan 67,4%. Kadar klorofil menurun sejalan dengan meningkatnya penambahan konsentrasi hidrolisat talas. Simpulan, penggunaan hidrolisat talas dapat meningkatkan kerapatan sel, berat segar dan berat kering, kadar lipida dan menurunkan kadar klorofil mikroalga T.chui pada kultur miksotrofik.

Kata Kunci:   Hidrolisat Talas, Lipida, Miksotrofik, Pertumbuhan, Tetraselmis chui

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Published
2021-03-20
How to Cite
Salim, M. (2021). Pemanfaatan Hidrolisat Talas (Colocasia esculenta L.) pada Kultur Miksotrofik Mikroalga Tetraselmis chui: Pengamatan Pertumbuhan dan Kadar Lipida. BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 4(1), 1-11. https://doi.org/https://doi.org/10.31539/bioedusains.v4i1.2117
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