Kandungan Logam Berat dan Vitamin E pada Testis Tikus Wistar setelah Dipaparkan Air Lindi
Abstract
This study aims to determine the heavy metal content and vitamin E concentration of male Wistar rat testes exposed to Sarimukti landfill leachate. The research design used was a randomized block design. Rats were divided into four treatments, namely: aquabidestylates, 63% leachate, CdCl2, H2O2. The test substance was administered intraperitoneally for 14 days. Heavy metal concentrations were measured using the Atomic Absorption Spectrometry (AAS) method, while Vitamin E concentrations were measured using HPLC. The results showed that the concentration of heavy metal Zn in testicular tissue exposed to 63% leachate was 0.6 ppm, aquabidestylates was 0.5 ppm, while the other six metals had a concentration of 0 ppm. The measurement results of Vitamin E in the 63% leachate treatment were meager, namely, 1.06 ppm when compared to the aquabidestyl treatment of 7.92 ppm, CdCl2 and H2O2 were 0.53 ppm and 0.85 ppm, respectively. In conclusion, Sarimukti landfill leachate can cause an increase in ROS, which is indicated by a biomarker of decreased Vitamin E concentration in the testes, causing testicular damage.
Keywords: Leachate, Heavy Metals, Wistar Rat Testicles, Vitamin E
References
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Nesta, B. S., Shouta, M. M. N., Yoshinori, I., Osei, A., Elvis, B., Hazuki, M., & Mayumi, I. (2016). Heavy Metals and Metalloid Accumulation in Livers and Kidneys of Wild Rats Around Gold-Mining Communities in Tarkwa, Ghana. Journal of Environmental Chemistry and Ecotoxicology, 8(7), 58–68. https://doi.org/10.5897/jece2016.0374
Oyelowo, O. T., Omamogho, O. E., & Ezenwajiaku, I. F. (2020). Heavy Metals Correlate with Cellular Adenosine Triphosphate and Fructose Levels in Municipal Dumpsite Exposure. Biokemistri, 32(1), 1–10. http://ojs.klobexjournals.com/index.php/bkr/article/view/459
Saito, H., Hara, K., Kitajima, S., & Tanemura, K. (2020). Effect of Vitamin E Deficiency on Spermatogenesis in Mice and Its Similarity to Aging. Reproductive Toxicology, 1-36. https://doi.org/10.1016/j.reprotox.2020.10.003
Seibert, D., Quesada, H., Bergamasco, R., Borba, F. H., & Pellenz, L. (2019). Presence of Endocrine Disrupting Chemicals in Sanitary Landfill Leachate, Its Treatment and Degradation by Fenton Based Processes: A review. Process Safety and Environmental Protection, 131, 255–267. https://doi.org/10.1016/j.psep.2019.09.022
Vaccari, M., Tudor, T., & Vinti, G. (2019). Characteristics of Leachate from Landfills and Dumpsites in Asia, Africa and Latin America : An Overview. Waste Management, 95, 416–431. https://doi.org/10.1016/j.wasman.2019.06.032
Wasiu, A. O., & Abdulfatai, A. (2019). Toxic Effects of Paraquat Dichloride Leachate on Testes and Sperm Parameters of Male Wistar Rats. International Journal of Anatomy and Research, 7(1.3), 6274–6279. https://doi.org/10.16965/ijar.2018.457
Xaypanya, P., Takemura, J., Chiemchaisri, C., Seingheng, H., & Tanchuling, M. A. N. (2018). Characterization of Landfill Leachates and Sediments in Major Cities of Indochina Peninsular Countries — Heavy Metal Partitioning in Municipal Solid Waste Leachate. Environments, 5(6), 1-24. https://doi.org/10.3390/environments5060065
Yakubu, M. T., & Omar, S. A. (2020). Impact of Groundwater Samples and Leachates from Gbagede Dumpsite, Amoyo, Kwara State, Nigeria, on Testes and Prostate of Male Wistar Rats: A Biochemical And Histological Study. Andrologia, 52(11), 1–15. https://doi.org/10.1111/and.13801
Adedara, I. A., Awogbindin, I. O., Adesina, A. A., Oyebiyi, O. O., Lawal, T. A., & Farombi, E. O. (2015). Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Archives of Environmental Contamination and Toxicology, 68(1), 74–82. https://doi.org/10.1007/s00244-014-0075-x
Ademola, O. J., Alimba, C. G., & Bakare, A. A. (2020). Reproductive Toxicity Assessment of Olusosun Municipal Landfill Leachate in Mus musculus using Abnormal Sperm Morphology and Dominant Lethal Mutation Assays. Environmental Health and Toxicology, 35(2). 1-10 https://doi.org/10.5620/eaht.e2020010
Akintunde, J. K., Oboh, G., & Akindahunsi, A. A. (2013). Testicular Membrane Lipid Damage by Complex Mixture of Leachate from Municipal Battery Recycling Site as Indication of Idiopathic Male Infertility in Rat. Interdisciplinary Toxicology, 6(4), 192–197. https://doi.org/10.2478/intox-2013-0028
Anbarkeh, F. R., Nikravesh, M. R., Jalali, M., Sadeghnia, H. R., & Sargazi, Z. (2019). The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and the Protective Effect of Vitamin E. International Journal of Fertility and Sterility, 13(2), 154–160. https://doi.org/10.22074/ijfs.2019.5612
Asadi, N., Bahmani, M., Kheradmand, A., & Rafieian-Kopaei, M. (2017). The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving It: A Review. Journal of Clinical and Diagnostic Research, 11(5), 1–5. https://doi.org/10.7860/JCDR/2017/23927.9886
Azevedo, M. M., Guimarães-Soares, L., Pascoal, C., & Cássio, F. (2016). Copper and Zinc Affect the Activity of Plasma Membrane H+-Atpase and Thiol Content in Aquatic Fungi. Microbiology (United Kingdom), 162(5), 740–747. https://doi.org/10.1099/mic.0.000262
Fang, J., Xie, S., Chen, Z., Wang, F., Chen, K., Zuo, Z., Cui, H., Guo, H., Ouyang, P., Chen, Z., Huang, C., Liu, W., & Geng, Y. (2021). Protective Effect of Vitamin E on Cadmium-Induced Renal Oxidative Damage and Apoptosis in Rats. Biological Trace Element Research. https://doi.org/10.1007/s12011-021-02606-4
Fu, Z., & Xi, S. (2019). The Effects of Heavy Metals on Human Metabolism. Toxicology Mechanisms and Methods, 30(3) 167–176. https://doi.org/10.1080/15376516.2019.1701594
Gandhi, J., Hernandez, R. J., Chen, A., Smith, N. L., Sheynkin, Y. R., Joshi, G., & Khan, S. A. (2017). Impaired Hypothalamic-Pituitary-Testicular Axis Activity, Spermatogenesis, and Sperm Function Promote Infertility in Males with Lead Poisoning. Zygote, 25(2), 103-110. https://doi.org/10.1017/S0967199417000028
He, Y., Zou, L., Luo, W., Yi, Z., Yang, P., Yu, S., Liu, N., Ji, J., Guo, Y., Liu, P., He, X., Lv, Z., & Huang, S. (2020). Heavy Metal Exposure, Oxidative Stress and Semen Quality: Exploring Associations and Mediation Effects in Reproductive-Aged Men. Chemosphere, 244, 1-8. https://doi.org/10.1016/j.chemosphere.2019.125498
Jomova, K., & Valko, M. (2011). Advances In Metal-Induced Oxidative Stress and Human Disease. Toxicology, 283(2–3), 65–87. https://doi.org/10.1016/j.tox.2011.03.001
Luo, H., Zeng, Y., Cheng, Y., He, D., & Pan, X. (2019). Recent Advances in Municipal Landfill Leachate: A Review Focusing on Its Characteristics, Treatment, and Toxicity Assessment. Science of the Total Environment, 1-105. https://doi.org/10.1016/j.scitotenv.2019.135468
Marreiro, D., Cruz, K., Morais, J., Beserra, J., Severo, J., & de Oliveira, A. (2017). Zinc and Oxidative Stress : Current Mechanisms, 6(2). 1-9. https://doi.org/10.3390/antiox6020024
Mumtaz, S., Ali, S., Khan, R., Shakir, H. A., & Tahir, H. M., Mumtaz, S., Andleeb, S. (2020). Therapeutic Role of Garlic and Vitamins C and E Against Toxicity Induced by Lead on Various Organs. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-020-07654-2
Nesta, B. S., Shouta, M. M. N., Yoshinori, I., Osei, A., Elvis, B., Hazuki, M., & Mayumi, I. (2016). Heavy Metals and Metalloid Accumulation in Livers and Kidneys of Wild Rats Around Gold-Mining Communities in Tarkwa, Ghana. Journal of Environmental Chemistry and Ecotoxicology, 8(7), 58–68. https://doi.org/10.5897/jece2016.0374
Oyelowo, O. T., Omamogho, O. E., & Ezenwajiaku, I. F. (2020). Heavy Metals Correlate with Cellular Adenosine Triphosphate and Fructose Levels in Municipal Dumpsite Exposure. Biokemistri, 32(1), 1–10. http://ojs.klobexjournals.com/index.php/bkr/article/view/459
Saito, H., Hara, K., Kitajima, S., & Tanemura, K. (2020). Effect of Vitamin E Deficiency on Spermatogenesis in Mice and Its Similarity to Aging. Reproductive Toxicology, 1-36. https://doi.org/10.1016/j.reprotox.2020.10.003
Seibert, D., Quesada, H., Bergamasco, R., Borba, F. H., & Pellenz, L. (2019). Presence of Endocrine Disrupting Chemicals in Sanitary Landfill Leachate, Its Treatment and Degradation by Fenton Based Processes: A review. Process Safety and Environmental Protection, 131, 255–267. https://doi.org/10.1016/j.psep.2019.09.022
Vaccari, M., Tudor, T., & Vinti, G. (2019). Characteristics of Leachate from Landfills and Dumpsites in Asia, Africa and Latin America : An Overview. Waste Management, 95, 416–431. https://doi.org/10.1016/j.wasman.2019.06.032
Wasiu, A. O., & Abdulfatai, A. (2019). Toxic Effects of Paraquat Dichloride Leachate on Testes and Sperm Parameters of Male Wistar Rats. International Journal of Anatomy and Research, 7(1.3), 6274–6279. https://doi.org/10.16965/ijar.2018.457
Xaypanya, P., Takemura, J., Chiemchaisri, C., Seingheng, H., & Tanchuling, M. A. N. (2018). Characterization of Landfill Leachates and Sediments in Major Cities of Indochina Peninsular Countries — Heavy Metal Partitioning in Municipal Solid Waste Leachate. Environments, 5(6), 1-24. https://doi.org/10.3390/environments5060065
Yakubu, M. T., & Omar, S. A. (2020). Impact of Groundwater Samples and Leachates from Gbagede Dumpsite, Amoyo, Kwara State, Nigeria, on Testes and Prostate of Male Wistar Rats: A Biochemical And Histological Study. Andrologia, 52(11), 1–15. https://doi.org/10.1111/and.13801
Published
2021-06-17
Section
Articles
