Uji In Vitro Aktivitas Imunomodulator Minyak Atsiri Serai Dapur (Cymbopogon citratus) terhadap Proliferasi Sel Limfosit Mencit
Abstract
This study aims to determine the immunomodulatory activity of lemongrass essential oil on the proliferation of mouse lymphocyte cells in vitro. The method used is steam distillation to obtain citronella essential oil. Isolation of lymphocyte cells was obtained from the spleen organ of Swiss-Webster strain mice which was carried out aseptically. The lymphocyte cell proliferation test was carried out using the MTT Assay method and the absorbance was measured using an ELISA reader at 550 nm. The results showed that the lemongrass essential oil contained positive alkaloids, flavonoids, saponins, and steroids/triterpenoids with an essential oil yield of 0.043%. The higher the volatile oil concentration, the higher the Optical Density (OD) value obtained, but the OD increase at a 50 to 100 g/mL concentration was not significantly different. In conclusion, lemongrass essential oil has immunomodulatory activity against lymphocyte cell proliferation in mice tested in vitro.
Keywords: Immunomodulator, Essential Oil, Lemongrass (Cymbopogon citratus), Immune System
References
Jalaluddin, J., Aji, A., & Nuriani, S. (2018). Pemanfaatan Minyak Sereh (Cymbopogon nardus L) sebagai Antioksidan pada Sabun Mandi Padat. Jurnal Teknologi Kimia Unimal, 7(1), 52–60. https://ojs.unimal.ac.id/jtk/article/view/1170
Pembudi, P. A. (2020). Pandemi COVID-19: Refleksi Pentingnya Optimasi Lahan Pekarangan sebagai Penyokong Kemandirian Pangan dan Kesehatan Keluarga. EnviroScienteae, 16(3), 408–423. https://ppjp.ulm.ac.id/journal/index.php/es/article/download/9683/6723
Pradani, F. Y., & Nurindra, R. W. (2017). Daya Proteksi Serai Dapur (Cymbopogon citratus) terhadap Nyamuk Aedes aegypti. Jurnal SPIRAKEL (Sarana Penyebaran Informasi Kegiatan Litbang), 9(2), 60–67. https://doi.org/10.22435/spirakel.v8i2.7648
Pramudita, M., Anggraini, D. D., Hidayat, N., Yuniardiningsih, E., Apriliyanti, M. D., Wangi, P., & Ma’rufi, I. (2020). Lumbung Pangan Sebagai Upaya Ketangguhan Pangan Masa Pandemi COVID-19 Desa Kabuaran Bondowoso. Multidisciplinary Journal, 3(1), 34–40. http://www.proceeding.unindra.ac.id/index.php/sinasis/article/view/5332
Rita, W. S., Vinapriliani, N. P. E., & Gunawan, I. W. G. (2018). Formulasi Sediaan Sabun Padat Minyak Atsiri Serai Dapur (Cymbopogon citratus DC.) sebagai Antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Cakra Kimia (Indonesian E-Journal of Applied Chemistry), 6(2), 152–160. https://ojs.unud.ac.id/index.php/cakra/article/download/46711/28156
Rosyanti, L., & Hadi, I. (2020). Respon Imunitas dan Badai Sitokin Severe Acute Respiratory Syndrome Corona Virus 2 Literatur Review. Jurnal Kesehatan Madani Medika, 11(02), 176–201. https://jurnalmadanimedika.ac.id/index.php/JMM/article/download/122/82/
Sufyan, S., Jayuska, A., & Destiarti, L. (2018). Bioaktivitas Minyak Atsiri Serai Dapur (Cymbopogon citratus (DC.) Stapf) terhadap Rayap (Coptotermes curvignathus sp). Jurnal Kimia Khatulistiwa, 7(3), 47–55. https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/25254
Ulfah, M., Cahyani, V. S. N., & Kinasih, I. (2017). Pengaruh Pemberian Seduhan Teh Daun Sirsak (Annona muricata L.) terhadap Aktivitas Fagositosis Sel Makrofag dan Proliferasi Sel Limfosit Mencit Galur Balb/C yang Diinduksi Vaksin Hepatitis B. Momentum, 13(2), 63–71. https://publikasiilmiah.unwahas.ac.id/index.php/MOMENTUM/article/download/2039/2069
Yanti, R., Nurdiawati, H., Cahyanto, M. N., & Pranoto, Y. (2020). Identifikasi Komponen dan Uji Potensi Anti Jamur Minyak Atsiri Serai Dapur (Cymbopogon citratus) terhadap Jamur Penghasil Aflatoksin. AGRITEKNO (Jurnal Teknologi Pertanian), 9(2), 72–80. https://doi.org/10.30598/jagritekno.2020.9.2.72
Zulfadhli, Z., Andila, I., Diana, F., & Rinawati, R. (2017). Pengaruh Ekstrak Batang Serai (Cymbopogon citratus) terhadap Pertumbuhan Bakteri Edwardsiella tarda Secara In Vitro. Jurnal Akuakultura, 1(1), 2579–4752. http://jurnal.utu.ac.id/jakultura/article/view/511/425
