Isolasi Bakteri Fiksasi Nitrogen dan Fosfat pada Limbah Cair Karet (Hevea brasiliensis)
Abstract
This research aims to determine which bacteria are capable of fixing nitrogen and dissolving phosphate in liquid rubber waste in Perdamean Village, Labuhanbatu Regency. The research method used was the description method with bacterial isolation techniques, then testing was carried out, namely the nitrogen fixation ability test and the phosphate solvent ability test. The results of the research showed that bacterial isolates capable of fixing nitrogen were found to have high absorbance values, namely 1,340 ABS. In the phosphate solubilizing ability test, there were 2 isolates that were unable to dissolve phosphate. In conclusion, isolating nitrogen and phosphate fixing bacteria in liquid rubber waste is a way to prevent pollution and destruction of the ecosystem in the environment.
Keywords: Nitrogen Fixation, Rubber Liquid Waste, Phosphate Solvents
References
Angraini, N., & Yanti, F. (2021). Penggunaan Spektrofotometer UV-Vis Untuk Analisis Nutrien Fosfat pada Sedimen dalam Rangka Pengembangan Modul Praktikum Oseanografi Kimia. Jurnal Penelitian Sains, 23(2), 78-83. https://doi.org/10.56064/jps.v23i2.620
Asril, M., & Lisafitri, Y. (2020). Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera. Jurnal Teknologi Lingkungan, 21(1), 40–48. https://doi.org/10.29122/jtl.v21i1.3743
Bulele, T., Rares, F. E. S., & Porotu’o, J. (2019). Identifikasi Bakteri dengan Pewarnaan Gram pada Penderita Infeksi Mata Luar di Rumah Sakit Mata Kota Manado. Jurnal E-Biomedik, 7(1), 30–36. https://doi.org/10.35790/ebm.7.1.2019.22820
Cheunbarn, T., Cheunbarn, S., & Klayrung, S. (2020). Isolation and Evaluation Efficiency of Bacteria in Rubber Processing Wastewater Treatment. The 12th NPRU National Academic Conference, 100–106. https://publication.npru.ac.th/handle/123456789/830
Ginting, T. S. M., Helmi, Z. T., Darmawi, Dewi, M., Hennivanda, Erina, & Daud, R. (2018). Isolasi dan Identifikasi Bakteri Gram Negatif pada Ambing Kambing Peranakan Etawa (PE). Jurnal Ilmiah Mahasiswa Veteriner, 2(3), 351–360. https://jim.unsyiah.ac.id/FKH/article/view/8206
Islam, H., Nelvia, N., & Zul, D. (2019). Isolasi dan Uji Potensi Bakteri Diazotrof Non Simbiotik Asal Tanah. Agroteknologi, 9(2), 35–40. https://ejournal.uin-suska.ac.id/index.php/agroteknologi/article/view/4508
Ismail, Y. S., Yulvizar, C., & Putriani. (2017). Isolation, Characterization, and Antimicrobial Activity of Lactic Acid Bacteria From the Fermented Cacao Seed (Theobroma cacao L.). Bioleuser, 1(2), 45–53.
Kosasi, C., Lolo, A. W., & Sedewi, S. (2019). Isolasi dan Uji Aktivitas Antibakteri dari Bakteri yang Berasosiasi dengan Alga Turbinaria ornata (Turner) J. Agardh serta Identifikasi secara Biokimia. Pharmacon–Program Studi Farmasi, Fmipa, Universitas Sam Ratulangi, 8(2), 351-359. https://doi.org/10.35799/pha.8.2019.29301
Laina, L., Mida, M., Roaini, R., & Kapli, H. (2018). Uji kualitas Air Sungai Sekitar Penduduk Desa Kebagusan Kec. Gedongtataan Kab. Pesawaran terhadap Limbah Cair di PTPN VII Way Berulu. Seminar Nasional Sains dan Teknologi Terapan, 131–135. http://semnas.radenfatah.ac.id/index.php/semnasfst/article/view/24
Panjaitan, F. J., Bachtiar, T., Arsyad, I., Lele, O. K., & Indriyani, W. (2020). Karakterisasi Mikroskopis dan Uji Biokimia Bakteri Pelarut Fosfat (BPF) dari Rhizosfer Tanaman Jagung Fase Vegetatif. Jurnal Ilmu Pertanian dan Lingkungan, 1(1), 9–17. https://stikessantupaulus.e-journal.id/ciwal/article/view/93
Rahmawati, L. R., Adlina, S., & Yuliana, A. (2021). Isolasi dan Identifikasi Bakteri Penghasil Protease Ekstraseluler dari Limbah Cair Tahu Putih. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu Keperawatan, Analis Kesehatan dan Farmasi, 21(2), 187–193. https://ejurnal.universitas-bth.ac.id/index.php/P3M_JKBTH/article/download/748/606
Rusdiansyah, A. I. (2018). Kumpulan Esai Inovatif. Jawa Barat: Guepedia
Sitorus, E. (2021). Proses Pengolahan Limbah. Medan: Yayasan Kita Menulis