Pengaruh Penambahan Variasi Konsentrasi Karbon Aktif pada Media Kultur In Vitro untuk Pertumbuhan Anggrek Dendrobium welirang
Abstract
This research aims to determine the effect of different concentrations of added activated carbon and determine the optimal concentration in the in vitro culture growing medium for Dendrobium welirang orchids to increase plant growth. The type of research used is true experimentation. This research used a Completely Randomized Design (CRD), and the sample used in this research was the orchid species Dendrobium welirang. The instrument used is in the form of a table. The type of data used is quantitative data. The data were analyzed statistically using the ANOVA test. Data collection was carried out in the third month after planting. The data taken includes leaf length, number of leaves, root length, and number of roots. The results of the research showed that adding variations in activated carbon concentration to the in vitro culture medium affected the parameters of root number, root length, leaf length, and number of leaves of the Dendrobium welirang orchid. In conclusion, the addition of activated carbon has a real influence on the growth of Dendrobium welirang orchids.
Keywords: Dendrobium welirang, Activated Carbon, In vitro Culture.
References
Ambarwati, I. D., Alfian, F. N., & Dewanti, P. (2021). Respon Anggrek Dendrobium sp., Oncidium sp., dan Phalaenopsis sp. Terhadap Pemberian Empat Jenis Nutrisi Organik yang Berbeda pada Tahap Regenerasi Planlet. Agrikultura, 32(1), 27. https://doi.org/10.24198/agrikultura.v32i1.32366
Bhattacharjee, D., & Hossain, M. (2015). Effects of Plant Growth Regulators And Explants on Propagation In The Monopodial And Sympodial Orchid: A Study In vitro. J. Orchid Soc., 29, 91–102. https://orchidsocietyindia.org/wp-content/uploads/2018/09/Journal-2015.pdf#page=88
Campbell, N. A., & Manalu, W. (2003). Biology (Ed. 5). http://opac.lib.ulm.ac.id/id/opac/detail.php?q1=574&q2=BIO&q3=2004&q4=979-688-470-4
E. F. George, P. D. S. (1984). Plant propagation by tissue culture : handbook and directory of commercial laboratories. Exegetics Ltd. https://books.google.co.id/books/about/Plant_Propagation_by_Tissue_Culture.html?id=rX8nzgEACAAJ&redir_esc=y
Fachrina Wibowo, Armaniar, N. A. (2023). Perbanyakan Vegetatif Tunas Mikro Anggrek Dendrobium (Dendrobium sp) Secara In vitro Dengan Pemberian Bap Dan Arang Aktif. 25(1), 910–916. http://dx.doi.org/10.37159/j. p agros.v25i1.2525
Ferziana. (2013). Pengaruh Pupuk Daun dan Arang Aktif pada Media Subkultur II terhadap Pertumbuhan Bibit Anggrek Phalaenopsis Effect of Foliar Fertilizers and Activated Charcoal on Media Subcultures II on Growth of Phalaenopsis Orchid Seed Ferziana. Jurnal Penelitian Pertanian Terapan, 13(3), 144–150. https://doi.org/https://doi.org/10.25181/jppt.v13i3.178
Haniawasniati, N., Nurokhman, A., Habisukan, U. H., Rahayu, S. C., Sari, V. W., Novitasari, L., Metalisa, E., & Indriani, A. (2022). Morfologi Eksplan Daun ( Folium ) Duku Terhadap Kultur Jaringan Pada Media Murashige and Skoog ( Ms ). 221–227. https://proceedings.radenfatah.ac.id/index.php/semnaspbio/article/view/705
Heriansyah, P., Sagiarti, T., Program Studi Agroteknologi, R., Pertanian Universitas Islam Kuantan Singingi, F., Kuantan Jln Gatot Subroto, T. K., Telp, J., & Kuantan, T. (2014). Pengaruh Pemberian Myoinositol dan Arang Atif pada Media Subkultur Jaringan Tanaman Anggrek (Dendrobium sp.). Jurnal Agroteknologi, 5(1), 9–16. https://doi.org/http://dx.doi.org/10.24014/ja.v5i1.1142
Ikenganyia, E., Anikwe, M., Omeje, T., & Adinde, J. (2017). Plant Tissue Culture Regeneration and Aseptic Techniques. Asian Journal of Biotechnology and Bioresource Technology, 1(3), 1–6. https://doi.org/10.9734/ajb2t/2017/31724
Ismaini, L. (2021). Pengaruh Komposisi Media Pada Perbanyakan Medinilla Beamanii Secara In vitro. Semnas Biologi Ke-9 Tahun 2021 FMIPA Universitas Negeri Semarang, 10–16. https://proceeding.unnes.ac.id/index.php/semnasbiologi/article/view/751
K, S., & Fathnur. (2018). Metodologi Penelitian Farmasi Komunitas dan Eksperimental. Deepublish. https://repo.stikes-ibnusina.ac.id/xmlui/handle/123456789/322
Khuraijam, J. S., Sharma, S. C., & Roy, R. K. (2017). Orchids: Potential ornamental crop in north india. International Journal of Horticultural & Crop Science Research, 7(1), 1–8. https://www.ripublication.com/ijhcsr17/ijhcsrv7n1_01.pdf
Lempang, M. (2014). Pembuatan dan Kegunaan Karbon Aktif. Jurnal Info Teknis EBONI, 11(2), 65–80. http://ejournal.forda-mof.org/ejournal-litbang/index.php/buleboni/article/view/5041/4463arang
Marwoto, D. B. (2004). Pengaruh Berbagai Sumber Arang dalam Media Kultur In vitro terhadap Pertumbuhan Plantlet Oncidium. Jurnal Hortikultura, 14(1), 1–5. http://124.81.126.59/handle/123456789/7935
Miranti, I. P., & Andriani, V. (2022). Aplikasi Sari Akar Eceng Gondok Pada Media Murashige And Skoog (MS) Sebagai Media Multiplikasi Tanaman Krisan (Chrysanthemum morifolium Ramat, Varietan Puspita Nusantara) Secara In vitro. Quagga: Jurnal Pendidikan Dan Biologi, 14(2), 169–174. https://doi.org/10.25134/quagga.v14i2.4500
Nisyawati, & Kariyana, K. (2013). Effect Of Ascorbic Acid, Activated Charcoal And Light Duration On Shoot Regeneration Of Banana Cultivar Barangan ( Musa Acuminata L .) In vitro Culture. In vitro, 15(April), 13–17. https://www.cabdirect.org/cabdirect/abstract/20133290250
Prassetio, A., Silvina, F., & Murniati. (2015). Respon Eksplan Duku (Lansium Domesticum Corr.) Terhadap Pemberian Auksin Dan Sitokinin Dalam Medium Murashige And Skoog Duku. 17(4), 306–314. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/5507/5385
Qomusuddin, I. F., & Romlah, S. (2022). Analisis Data Kuantitatif dengan Program IBM SPSS Statistic 20.0 (p. 125). Depublish Publisher. https://books.google.co.id/books?id=P3tmEAAAQBAJ&lpg=PP1&ots=HOQolLvY2l&dq=data kuantitatif adalah &lr&hl=id&pg=PR4#v=onepage&q=data kuantitatif adalah&f=false
Sakularat, S., Tiwa, R., & Sorapong, B. (2015). Influence of different type of Culture Media and activated charcoal on Callus Induction and ShootMultiplication of Cadaminelyrata Sakularat. Journal of Agricultural Technology, 11(8), 1697–1704. www.journal.uta45jakarta.ac.id
Sandra, E. (2013). Cara mudah memahami dan menguasai kultur jaringan skala rumah tangga (p. 124). IPB Press. https://sip.unper.ac.id/index.php?p=show_detail&id=3107
Sari, D. I., Suwirmen, & Nasir, N. (2015). Pengaruh Konsentrasi Thidiazuron (TDZ) dan Arang Aktif pada Sub Kultur Tunas Pisang Kepok Hijau (Musa paradisiaca L.). Jurnal Online Ilmu Pengetahuan Alam, 4(3), 280–289. http://jurnal.untad.ac.id/jurnal/index.php/ejurnalfmipa/article/view/5133
Silva, J. A. T. da. (2013). Ammonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium. Journal of Ornamental Plants (Journal of Ornamental and Horticultural Plants), 3(3), 155–160. https://www.researchgate.net/publication/280494138_Ammonium_to_Nitrate_Ratio_Affects_Protocorm_Like_Bodies_PLB_Formation_In_vitro_of_Hybrid_Cymbidium
Sucandra, A., Silvina, F., Arnis, D., & Yulia, E. (2015). UJi Pemberian Beberapa Konsentrasi Glisin pada Media Vacin And Went (VW) terhadap Pertumbuhan Plantlet Anggrek (Dendrobium Sp.) secara In vitro. Jom Faperta, 2(1), 1–11. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/5849
Sudrajad, H., Suharto, D., & Rahmawati Wijaya, N. (2016). Inisiasi Kalus Sanrego (Lunasia Amara Blanco.) dalam Kultur Jaringan. Proceeding Biology Education Conference, 13(1), 619–623. https://simdos.unud.ac.id/uploads/file_pendidikan_1_dir/ddeec13c19c352d21ccca286966a08ec.pdf
Sugiyono. (2018). Metode penelitian manajemen : Pendekatan kuantitatif, kualitatif, kombinasi ( mixed methods ), penelitian tindakan ( action research ), penelitian evaluasi (Setiyawarni (ed.)). https://inlislite.uin-suska.ac.id/opac/detail-opac?id=27802
Suranthran, P., Sinniah, U. R., Sreeramanan Subramaniam, M. A. A., Gantait, & Saikat, N. R. and. (2011). Effect of activated charcoal, culture media and plant growth regulators on in vitro germination and development of elite dura oil palm (Elaeis guineensis Jacq.) zygotic embryos. Plant Cell Biotechnology and Molecular Biology, 20(7–8), 314–323. https://doi.org/10.5897/AJB11.964
Thomas, T. D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26(6), 618–631. https://doi.org/10.1016/j.biotechadv.2008.08.003
Widiastoeti, D., Santi, A., & Solvia, N. (2013). Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In vitro. Jurnal Hortikultura, 22(3), 205. https://doi.org/10.21082/jhort.v22n3.2012.p205-209
Widiastoety, D. (2014). Effect of Auxin and Cytokinin on the Growth of Mokara Orchid Plantlets. Jurnal Hortikultura, 24(3), 230–238. https://dx.doi.org/10.21082/jhort.v24n3.2014.p230-238
Yusron, Tri Nopsagiarti. (2020). Respon Pertumbuhan Eksplan Jeruk Kasturi (Citrus Microcarpa) Terhadap Pemberian Benzyl Amino Purin (Bap) Dan Arang Aktif Pada Media MS. Jurnal Agro Indragiri, 6(2), 1–16. https://doi.org/10.32520/jai.v6i2.1471
Zulkarnain. (2011). Kultur Jaringan Tanaman: Solusi Perbanyakan Tanaman Budi Daya, (1st ed.). Jakarta : Bumi Aksara
Bhattacharjee, D., & Hossain, M. (2015). Effects of Plant Growth Regulators And Explants on Propagation In The Monopodial And Sympodial Orchid: A Study In vitro. J. Orchid Soc., 29, 91–102. https://orchidsocietyindia.org/wp-content/uploads/2018/09/Journal-2015.pdf#page=88
Campbell, N. A., & Manalu, W. (2003). Biology (Ed. 5). http://opac.lib.ulm.ac.id/id/opac/detail.php?q1=574&q2=BIO&q3=2004&q4=979-688-470-4
E. F. George, P. D. S. (1984). Plant propagation by tissue culture : handbook and directory of commercial laboratories. Exegetics Ltd. https://books.google.co.id/books/about/Plant_Propagation_by_Tissue_Culture.html?id=rX8nzgEACAAJ&redir_esc=y
Fachrina Wibowo, Armaniar, N. A. (2023). Perbanyakan Vegetatif Tunas Mikro Anggrek Dendrobium (Dendrobium sp) Secara In vitro Dengan Pemberian Bap Dan Arang Aktif. 25(1), 910–916. http://dx.doi.org/10.37159/j. p agros.v25i1.2525
Ferziana. (2013). Pengaruh Pupuk Daun dan Arang Aktif pada Media Subkultur II terhadap Pertumbuhan Bibit Anggrek Phalaenopsis Effect of Foliar Fertilizers and Activated Charcoal on Media Subcultures II on Growth of Phalaenopsis Orchid Seed Ferziana. Jurnal Penelitian Pertanian Terapan, 13(3), 144–150. https://doi.org/https://doi.org/10.25181/jppt.v13i3.178
Haniawasniati, N., Nurokhman, A., Habisukan, U. H., Rahayu, S. C., Sari, V. W., Novitasari, L., Metalisa, E., & Indriani, A. (2022). Morfologi Eksplan Daun ( Folium ) Duku Terhadap Kultur Jaringan Pada Media Murashige and Skoog ( Ms ). 221–227. https://proceedings.radenfatah.ac.id/index.php/semnaspbio/article/view/705
Heriansyah, P., Sagiarti, T., Program Studi Agroteknologi, R., Pertanian Universitas Islam Kuantan Singingi, F., Kuantan Jln Gatot Subroto, T. K., Telp, J., & Kuantan, T. (2014). Pengaruh Pemberian Myoinositol dan Arang Atif pada Media Subkultur Jaringan Tanaman Anggrek (Dendrobium sp.). Jurnal Agroteknologi, 5(1), 9–16. https://doi.org/http://dx.doi.org/10.24014/ja.v5i1.1142
Ikenganyia, E., Anikwe, M., Omeje, T., & Adinde, J. (2017). Plant Tissue Culture Regeneration and Aseptic Techniques. Asian Journal of Biotechnology and Bioresource Technology, 1(3), 1–6. https://doi.org/10.9734/ajb2t/2017/31724
Ismaini, L. (2021). Pengaruh Komposisi Media Pada Perbanyakan Medinilla Beamanii Secara In vitro. Semnas Biologi Ke-9 Tahun 2021 FMIPA Universitas Negeri Semarang, 10–16. https://proceeding.unnes.ac.id/index.php/semnasbiologi/article/view/751
K, S., & Fathnur. (2018). Metodologi Penelitian Farmasi Komunitas dan Eksperimental. Deepublish. https://repo.stikes-ibnusina.ac.id/xmlui/handle/123456789/322
Khuraijam, J. S., Sharma, S. C., & Roy, R. K. (2017). Orchids: Potential ornamental crop in north india. International Journal of Horticultural & Crop Science Research, 7(1), 1–8. https://www.ripublication.com/ijhcsr17/ijhcsrv7n1_01.pdf
Lempang, M. (2014). Pembuatan dan Kegunaan Karbon Aktif. Jurnal Info Teknis EBONI, 11(2), 65–80. http://ejournal.forda-mof.org/ejournal-litbang/index.php/buleboni/article/view/5041/4463arang
Marwoto, D. B. (2004). Pengaruh Berbagai Sumber Arang dalam Media Kultur In vitro terhadap Pertumbuhan Plantlet Oncidium. Jurnal Hortikultura, 14(1), 1–5. http://124.81.126.59/handle/123456789/7935
Miranti, I. P., & Andriani, V. (2022). Aplikasi Sari Akar Eceng Gondok Pada Media Murashige And Skoog (MS) Sebagai Media Multiplikasi Tanaman Krisan (Chrysanthemum morifolium Ramat, Varietan Puspita Nusantara) Secara In vitro. Quagga: Jurnal Pendidikan Dan Biologi, 14(2), 169–174. https://doi.org/10.25134/quagga.v14i2.4500
Nisyawati, & Kariyana, K. (2013). Effect Of Ascorbic Acid, Activated Charcoal And Light Duration On Shoot Regeneration Of Banana Cultivar Barangan ( Musa Acuminata L .) In vitro Culture. In vitro, 15(April), 13–17. https://www.cabdirect.org/cabdirect/abstract/20133290250
Prassetio, A., Silvina, F., & Murniati. (2015). Respon Eksplan Duku (Lansium Domesticum Corr.) Terhadap Pemberian Auksin Dan Sitokinin Dalam Medium Murashige And Skoog Duku. 17(4), 306–314. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/5507/5385
Qomusuddin, I. F., & Romlah, S. (2022). Analisis Data Kuantitatif dengan Program IBM SPSS Statistic 20.0 (p. 125). Depublish Publisher. https://books.google.co.id/books?id=P3tmEAAAQBAJ&lpg=PP1&ots=HOQolLvY2l&dq=data kuantitatif adalah &lr&hl=id&pg=PR4#v=onepage&q=data kuantitatif adalah&f=false
Sakularat, S., Tiwa, R., & Sorapong, B. (2015). Influence of different type of Culture Media and activated charcoal on Callus Induction and ShootMultiplication of Cadaminelyrata Sakularat. Journal of Agricultural Technology, 11(8), 1697–1704. www.journal.uta45jakarta.ac.id
Sandra, E. (2013). Cara mudah memahami dan menguasai kultur jaringan skala rumah tangga (p. 124). IPB Press. https://sip.unper.ac.id/index.php?p=show_detail&id=3107
Sari, D. I., Suwirmen, & Nasir, N. (2015). Pengaruh Konsentrasi Thidiazuron (TDZ) dan Arang Aktif pada Sub Kultur Tunas Pisang Kepok Hijau (Musa paradisiaca L.). Jurnal Online Ilmu Pengetahuan Alam, 4(3), 280–289. http://jurnal.untad.ac.id/jurnal/index.php/ejurnalfmipa/article/view/5133
Silva, J. A. T. da. (2013). Ammonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium. Journal of Ornamental Plants (Journal of Ornamental and Horticultural Plants), 3(3), 155–160. https://www.researchgate.net/publication/280494138_Ammonium_to_Nitrate_Ratio_Affects_Protocorm_Like_Bodies_PLB_Formation_In_vitro_of_Hybrid_Cymbidium
Sucandra, A., Silvina, F., Arnis, D., & Yulia, E. (2015). UJi Pemberian Beberapa Konsentrasi Glisin pada Media Vacin And Went (VW) terhadap Pertumbuhan Plantlet Anggrek (Dendrobium Sp.) secara In vitro. Jom Faperta, 2(1), 1–11. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/5849
Sudrajad, H., Suharto, D., & Rahmawati Wijaya, N. (2016). Inisiasi Kalus Sanrego (Lunasia Amara Blanco.) dalam Kultur Jaringan. Proceeding Biology Education Conference, 13(1), 619–623. https://simdos.unud.ac.id/uploads/file_pendidikan_1_dir/ddeec13c19c352d21ccca286966a08ec.pdf
Sugiyono. (2018). Metode penelitian manajemen : Pendekatan kuantitatif, kualitatif, kombinasi ( mixed methods ), penelitian tindakan ( action research ), penelitian evaluasi (Setiyawarni (ed.)). https://inlislite.uin-suska.ac.id/opac/detail-opac?id=27802
Suranthran, P., Sinniah, U. R., Sreeramanan Subramaniam, M. A. A., Gantait, & Saikat, N. R. and. (2011). Effect of activated charcoal, culture media and plant growth regulators on in vitro germination and development of elite dura oil palm (Elaeis guineensis Jacq.) zygotic embryos. Plant Cell Biotechnology and Molecular Biology, 20(7–8), 314–323. https://doi.org/10.5897/AJB11.964
Thomas, T. D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26(6), 618–631. https://doi.org/10.1016/j.biotechadv.2008.08.003
Widiastoeti, D., Santi, A., & Solvia, N. (2013). Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In vitro. Jurnal Hortikultura, 22(3), 205. https://doi.org/10.21082/jhort.v22n3.2012.p205-209
Widiastoety, D. (2014). Effect of Auxin and Cytokinin on the Growth of Mokara Orchid Plantlets. Jurnal Hortikultura, 24(3), 230–238. https://dx.doi.org/10.21082/jhort.v24n3.2014.p230-238
Yusron, Tri Nopsagiarti. (2020). Respon Pertumbuhan Eksplan Jeruk Kasturi (Citrus Microcarpa) Terhadap Pemberian Benzyl Amino Purin (Bap) Dan Arang Aktif Pada Media MS. Jurnal Agro Indragiri, 6(2), 1–16. https://doi.org/10.32520/jai.v6i2.1471
Zulkarnain. (2011). Kultur Jaringan Tanaman: Solusi Perbanyakan Tanaman Budi Daya, (1st ed.). Jakarta : Bumi Aksara
Published
2024-06-30
Section
Articles
Copyright (c) 2024 Andien Narita Putri Warisman, Praptining Rahayu, Eko Retno Mulyaningrum
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
